FastQCFastQC Report
Sun 2 Jul 2023
SRR1190359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1190359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216863316
Total Bases10.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGA22218861.0245559465668228RNA PCR Primer, Index 1 (100% over 22bp)
ATACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTC20112970.9274491588056322RNA PCR Primer, Index 1 (100% over 26bp)
TGCCAAGGACGTTTTCATTAATCTGGAATTCTCGGGTGCCAAGGAACTCC18456540.8510678680206107RNA PCR Primer, Index 1 (100% over 27bp)
AACTTTTCGAATCGCATGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG15740820.7258406027509051RNA PCR Primer, Index 1 (100% over 29bp)
TATACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACT14575900.6721238183040603RNA PCR Primer, Index 1 (100% over 25bp)
ACGGGGAAACTCACCAGGTCCAGACTGGAATTCTCGGGTGCCAAGGAACT14012450.646142015093046RNA PCR Primer, Index 1 (100% over 25bp)
TGCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTGCCA13830700.6377611601217055No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCAA13734910.6333440921838528No Hit
AAGGCTGAAACTTAAAGGAATTGACTGGAATTCTCGGGTGCCAAGGAACT13533690.6240654366827075RNA PCR Primer, Index 1 (100% over 25bp)
AAGGATTGGCTCTAAGGGTCGGTGGAATTCTCGGGTGCCAAGGAACTCCA13468310.6210506344927419RNA PCR Primer, Index 1 (100% over 28bp)
ATTTGAATGAAGAGCTTTTAGTGGGCCTGGAATTCTCGGGTGCCAAGGAA13234510.6102696502159913RNA PCR Primer, Index 1 (100% over 23bp)
AAGGGGAATCTGACTGTCTAATTAAAACTGGAATTCTCGGGTGCCAAGGA12329740.5685489010967627RNA PCR Primer, Index 1 (100% over 22bp)
AGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG12034860.5549513962057097RNA PCR Primer, Index 1 (100% over 29bp)
AATGACCCACTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG11827080.5453702460216923RNA PCR Primer, Index 11 (100% over 35bp)
AGATGTCCTAAGGGGGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT11732670.5410168126360292RNA PCR Primer, Index 1 (100% over 30bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA11007230.5075653274618378No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCTTGGAATTCTCGGGTGCCAA10278920.47398150086388974No Hit
AATAACTTTTCGAATCGCATGGCCTGGAATTCTCGGGTGCCAAGGAACTC9995410.46090828934848527RNA PCR Primer, Index 1 (100% over 26bp)
TGCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGGGTGCCAAGG9954260.45901078078138396Illumina Small RNA Adapter 2 (100% over 21bp)
AGAAATCTCCAGTAGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA9793790.45161118904960396RNA PCR Primer, Index 1 (100% over 32bp)
TAACGATCAACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCA9088200.4190750269630664RNA PCR Primer, Index 1 (100% over 28bp)
AAGGGGAATCTGACTGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC9015530.41572406833436043RNA PCR Primer, Index 1 (100% over 31bp)
AATGACCCACTCGGCACCTTACGAGAAATCTGGAATTCTCGGGTGCCAAG8660150.3993367877857221No Hit
AGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT8390170.38688747155374126RNA PCR Primer, Index 1 (100% over 30bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGG8118120.37434270349347604Illumina Small RNA Adapter 2 (100% over 21bp)
ATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC7982150.36807285562303216RNA PCR Primer, Index 1 (100% over 33bp)
CGCGCAGGTTCGAGTCCTGCAGTTGTCGCCTGGAATTCTCGGGTGCCAAG7825900.36086785650736797No Hit
TGCCAAGGACGTTTTCATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC7338590.3383970205454204RNA PCR Primer, Index 1 (100% over 31bp)
AACGATCAACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG7148360.32962513586207454RNA PCR Primer, Index 1 (100% over 29bp)
AATGACCCACTCGGCACCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT7011660.32332162623576227RNA PCR Primer, Index 1 (100% over 30bp)
AACGGGGTATTGTAAGCAGTAGAGTAGCCTTGGAATTCTCGGGTGCCAAG6569810.30294704153652247No Hit
TACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCC6301820.29058948817327873RNA PCR Primer, Index 1 (100% over 27bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG6009840.2771257080658123Illumina Small RNA Adapter 2 (100% over 21bp)
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAA5959860.27482103058868657No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAATGGAATTCTCGGGTGCCAA5911280.2725809099036372No Hit
AGAACTGGCGATGCGGGATGAACCGAACTGGAATTCTCGGGTGCCAAGGA5886580.271441943643433RNA PCR Primer, Index 1 (100% over 22bp)
TTAACGATCAACTTAGAACTGGTTGGAATTCTCGGGTGCCAAGGAACTCC5853970.2699382315079974RNA PCR Primer, Index 1 (100% over 27bp)
ATGGAGAACAGAAATCTCCAGTAGAACTGGAATTCTCGGGTGCCAAGGAA5835610.26909161529191045RNA PCR Primer, Index 1 (100% over 23bp)
AGAACTGGTACGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG5687170.26224675085204363RNA PCR Primer, Index 11 (100% over 35bp)
CGTGAATCATCGAATCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAACT5630240.25962159501425314RNA PCR Primer, Index 1 (100% over 25bp)
AGAATTCGGTAAGCGTTGGATTGTTCTGGAATTCTCGGGTGCCAAGGAAC5485670.25295518399248307RNA PCR Primer, Index 1 (100% over 24bp)
AAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAAC5481790.2527762694544429RNA PCR Primer, Index 1 (100% over 24bp)
TTGCTGGCTCCGGTGCGCTTGTGACTGGAATTCTCGGGTGCCAAGGAACT5368600.24755685281506995RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGGTAAGAGCCTGACCGAGTTGGAATTCTCGGGTGCCAAGGAACTC5181460.23892745419423542RNA PCR Primer, Index 1 (100% over 26bp)
GCCAAGGACGTTTTCATTAATCTGGAATTCTCGGGTGCCAAGGAACTCCA5154960.23770548634421876RNA PCR Primer, Index 1 (100% over 28bp)
ACAGTGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA5125090.23632812107327547RNA PCR Primer, Index 1 (100% over 28bp)
AGGGTGAGAATCCCGTGTGGCGAGGAGTGTGGAATTCTCGGGTGCCAAGG4999580.23054060466363063Illumina Small RNA Adapter 2 (100% over 21bp)
ACTATGCCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA4672900.2154767383525575RNA PCR Primer, Index 11 (100% over 38bp)
ACGGGGAAACTCACCAGGTCCAGACACTGGAATTCTCGGGTGCCAAGGAA4605980.2123909236913079RNA PCR Primer, Index 1 (100% over 23bp)
TCCGTGATAGTTTAATGGTCAGAATGGGCTGGAATTCTCGGGTGCCAAGG4454550.20540818438836375Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAA4297080.19814692863960448RNA PCR Primer, Index 1 (100% over 23bp)
AGCTGTTGGGACCCGAAAGATGGTGAACTTGGAATTCTCGGGTGCCAAGG4293730.19799245345856467Illumina Small RNA Adapter 2 (100% over 21bp)
AACTATGCCGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCT4164640.1920398561091817RNA PCR Primer, Index 11 (100% over 37bp)
AACAACGGATCTCTTGGTTCTCGCTGGAATTCTCGGGTGCCAAGGAACTC4096550.18890009041455402RNA PCR Primer, Index 1 (100% over 26bp)
TGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCAA4043270.1864432433561055No Hit
CGGAGAGGGAGCCTGAGAAACGGCTACCACTGGAATTCTCGGGTGCCAAG4019160.185331483172562No Hit
AATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG3958520.1825352518357692RNA PCR Primer, Index 6 (100% over 34bp)
AACTTAGAACTGGTACGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT3909550.18027714747292714RNA PCR Primer, Index 1 (100% over 30bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAG3865710.17825559764105056No Hit
TTCCAGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC3853410.17768842011066546RNA PCR Primer, Index 11 (100% over 39bp)
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT3851890.1776183298792683RNA PCR Primer, Index 1 (100% over 25bp)
ACGCGCGCTACACTGACGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC3782030.17439694595465838RNA PCR Primer, Index 1 (100% over 27bp)
AATAACTTTTCGAATCGCATTGGAATTCTCGGGTGCCAAGGAACTCCAGT3696830.17046820403686902RNA PCR Primer, Index 1 (100% over 30bp)
AGGATAAATCCATAGGAATGTAGCTTGCCTTGGAATTCTCGGGTGCCAAG3596780.16585469900312694No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGGA3520140.1623206757568901RNA PCR Primer, Index 1 (100% over 22bp)
ATAGCTGTTGGGACCCGAAAGATGGTGAACTTGGAATTCTCGGGTGCCAA3498130.1613057507614612No Hit
AAGGGGAATCTGACTGTCTAATTAAAACATTGGAATTCTCGGGTGCCAAG3480620.1604983297405634No Hit
ACGGGGAAACTCACCAGGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG3318800.15303648681642404RNA PCR Primer, Index 1 (100% over 29bp)
ATTCCAGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTA3261610.15039934186010512RNA PCR Primer, Index 11 (100% over 38bp)
CTTGCGTGCCTTGTTGTAGACGGCCTTGGTGGAATTCTCGGGTGCCAAGG3258160.15024025547963124Illumina Small RNA Adapter 2 (100% over 21bp)
AACGAACGAGACCTTAACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT3240290.14941623414077093RNA PCR Primer, Index 1 (100% over 30bp)
ACTGTGGTAATTCTAGAGCTAATACATGCTTGGAATTCTCGGGTGCCAAG3201780.1476404612387279No Hit
ATTGCGATGGTCAGAAAGTGATGTTGACGCTGGAATTCTCGGGTGCCAAG3179280.14660294136607227No Hit
AAGCGTTGGATTGTTCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3175670.14643647706650395RNA PCR Primer, Index 1 (100% over 30bp)
AGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAAGGAACTC3155630.14551239269992533RNA PCR Primer, Index 1 (100% over 26bp)
GTGAATCATCGAATCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAACTC2851760.13150034097975336RNA PCR Primer, Index 1 (100% over 26bp)
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTTGGAATTCTCGGGTGCCAA2779350.1281613714695758No Hit
ACTCTACCGTCAGGGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC2734120.12607572596556627RNA PCR Primer, Index 1 (100% over 31bp)
AACGCAGATGTCCTAAGGGGGGCTCTGGAATTCTCGGGTGCCAAGGAACT2716060.12524294334778133RNA PCR Primer, Index 1 (100% over 25bp)
AAGCAGTAGAGTAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2659850.12265098814591585RNA PCR Primer, Index 1 (100% over 33bp)
AACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2630150.1212814619140104RNA PCR Primer, Index 1 (100% over 29bp)
AAACTTTCAACAACGGATCTCTTGGTTCTCGCTGGAATTCTCGGGTGCCA2514480.11594768752867358No Hit
AACTGTAGTTAAGCTGGTAAGAGCCTGACTGGAATTCTCGGGTGCCAAGG2494100.11500792508401929Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCGGAGAGGGAGCCTGAGAAACGGCTTGGAATTCTCGGGTGCCAAGGA2476490.11419589286368746RNA PCR Primer, Index 1 (100% over 22bp)
CGGGATAAGGATTGGCTCTAAGGGTCGGTGGAATTCTCGGGTGCCAAGGA2470520.11392060425747616RNA PCR Primer, Index 1 (100% over 22bp)
AGTTTTGGTGGCAGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2455750.11323953010107066RNA PCR Primer, Index 1 (100% over 33bp)
GATAACGCAGATGTCCTAAGGGGGGCTCTGGAATTCTCGGGTGCCAAGGA2454170.11316667315001308RNA PCR Primer, Index 1 (100% over 22bp)
AATGACCCACTCGGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2439440.11248744347338117RNA PCR Primer, Index 1 (100% over 33bp)
TAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAATTCTCGGGTGCCA2413170.11127608138206278No Hit
ATAACTGTGGTAATTCTAGAGCTAATACATGCTTGGAATTCTCGGGTGCC2400480.11069092017388502No Hit
GTTACGATCTGCTGAGATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2392740.11033401333769147RNA PCR Primer, Index 1 (100% over 31bp)
AGTTAAGCTGGTAAGAGCCTGACTGGAATTCTCGGGTGCCAAGGAACTCC2328980.10739391257855709RNA PCR Primer, Index 1 (100% over 27bp)
AATAGTAATTGAACTTAGTACGAGAGGAACTGGAATTCTCGGGTGCCAAG2292270.10570114126632647No Hit
ATAATAACTTTTCGAATCGCATGGCCTGGAATTCTCGGGTGCCAAGGAAC2204680.10166219168206393RNA PCR Primer, Index 1 (100% over 24bp)
ATTGTAAGCAGTAGAGTAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCA2197770.1013435578011728RNA PCR Primer, Index 1 (100% over 28bp)
CCGTGAATCATCGAATCTTTGAACGCTGGAATTCTCGGGTGCCAAGGAAC2197430.10132787972309709RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)