FastQCFastQC Report
Wed 30 Aug 2023
SRR11915743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11915743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18495146
Total Bases924.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6492903.5105967803660487Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1528440.8264006134366281No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA1505750.8141325296918446No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA1353660.731900142880732No Hit
AAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1324320.7160365211499277No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT1320070.7137386209332979No Hit
ACAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGA1033210.5586384665468442No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA1026390.5549510125521583No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA1010630.546429857866491No Hit
AAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1006220.5440454484652352No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC982340.5311339526597952No Hit
TCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGG981580.5307230340328214No Hit
AACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCG981330.530587863431843No Hit
AAAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC963530.5209637166421935No Hit
AAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG844930.456838783538124No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC716690.3875016720603341No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA693030.374709126383755No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA662680.35829941542499855No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC618640.33448776235667455No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG596060.3222791536763213Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC595550.32200340565032576No Hit
TAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG584480.31601805143900996No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC579840.31350928508485415Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA555210.30019227747647953No Hit
TAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT548630.29663458725873265No Hit
GAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAA541030.29252540098899465No Hit
TCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAATAGATCGGAAGAGCA505990.27357988955588675No Hit
AGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGAAG495170.2677297059455492No Hit
TAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC489720.26478298684422386No Hit
TCAAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA475570.2571323308288564No Hit
AAAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG466140.2520336957599578No Hit
TCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG459220.24829217352488053No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT444120.24012786922579576No Hit
TAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCAAT433990.23465075647415815No Hit
AGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCAATA410620.22201500869471374No Hit
TCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGA405870.2194467672761275No Hit
TTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGAG397040.21467254164957658No Hit
TAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAA393070.21252603250604238No Hit
TTGGGTTCTACTCTGCTGGCCTGTAGGCACCATCAATAGATCGGAAGAGC378320.20455096704832718No Hit
TCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATA374720.20260451039424074No Hit
TCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGA372350.20132309309696717No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCACTGTAGGCACC359970.1946294449365255No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC358270.1937102848498736Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGCTGTAGGCACCATCA349270.1888441432146575No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA346610.1874059280202492No Hit
AGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCGGAA337280.18236136119174187No Hit
TAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCA333830.18049600689824238No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT332160.17959306728370783Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGATCCCGTCATCACCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC330860.17889018015862107No Hit
ACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCGG326400.17647873663716956No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT324320.17535411723703073No Hit
TCGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA312710.169076794527602No Hit
AACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAATAGATCGGA311480.16841175517078913No Hit
AAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGA306160.16553532478197253No Hit
ATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGA305410.16512981297903787No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGCTGTAGGCACCATCAATAGATC304860.16483243765688577No Hit
TGGGGCTACAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATA297370.16078272645157815No Hit
TAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATC293950.15893359263019605No Hit
ACAGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA288860.15618151919427942No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCCTGTAGGCACCATC284800.15398634863439303No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAGATCGGA277620.15010424897429844No Hit
CCTTGGTTCGATTCCGAGTCCGGGCCTGTAGGCACCATCAATAGATCGGA270170.14607616506514737No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAAT264890.14322136197248728No Hit
ACAGCTCAGCTGGGAGAGCGCTTGCCTGTAGGCACCATCAATAGATCGGA258180.13959338304223173No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCACCACTGTAGGCAC256240.1385444591786407No Hit
TTGGGTTCTACTCTGCTGGCAGGCTGTAGGCACCATCAATAGATCGGAAG254970.13785779252567132No Hit
ATGTAGGTGAAGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC253900.13727926235348453No Hit
TGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGA252890.13673317312553251No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT251540.1360032518802501No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG250100.13522466921861553No Hit
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT239510.12949884256117794Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC236080.1276443019157567No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGTAGGCACC215050.1162737509614685No Hit
TTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAAT213730.11556005018830345No Hit
TAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCCTGTAGGCACCATC211250.11421915782659949No Hit
AGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAAT209550.11329999773994756No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCCTGTAGGCACCATC209170.11309453842646064No Hit
TAAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC206190.11148330486280023No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAA204410.11052089018383526No Hit
TAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCAAT203880.11023432850976143No Hit
TGATCCCGTCATCACCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA201400.10889343614805744No Hit
AGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC200450.10837978786434017No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCCTGTAGGCACCATCAA193080.10439495854750212No Hit
AGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA188130.1017185806481333No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)