FastQCFastQC Report
Wed 30 Aug 2023
SRR11915744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11915744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9760065
Total Bases488 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5250025.379083028647862Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA827790.8481398433309615No Hit
AAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG798840.8184781556270374No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA756530.7751280344956719No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA703380.7206714299546161No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT687290.7041858840079446No Hit
AACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCG640450.6561944003446699No Hit
TCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC620980.636245762707523No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA586140.6005492791287763No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA546680.5601192205174863No Hit
AAAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC538910.5521582079627544No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC527080.5400373870460904No Hit
AAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT507550.5200272744085208No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC477850.4895971491993137No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA416050.42627789876399386No Hit
ACAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGA377570.3868519318262737No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC348200.35675991911939114Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGAAG334820.3430509940251423No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG333200.34139116901373096Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA319850.3277129814196934No Hit
AGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCAATA317370.3251720147355576No Hit
AAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG308640.3162274021740634No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC308330.3159097813385464No Hit
TCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG277980.28481367695809406No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC276950.2837583561175054Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAA260360.26676051850064525No Hit
TCGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC244610.25062333088970207No Hit
AGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCGGAA226520.23208861826227592No Hit
TGGGGCTACAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATA212610.21783666399762705No Hit
AACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAATAGATCGGA208730.21386128063696297No Hit
TCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAATAGATCGGAAGAGCA207470.21257030562808749No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAGATCGGA206910.2119965389574762No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGTAGGCACC205440.2104904014471215No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT202260.20723222642472153Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCA197280.2021298013896424No Hit
TAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCAAT192900.1976421263587896No Hit
TAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAA192450.1971810638556198No Hit
TCAAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA190800.1954905013439972No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCACTGTAGGCACC185610.19017291380743878No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT185610.19017291380743878No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAA181850.18632048044761998No Hit
TTGGGTTCTACTCTGCTGGCCTGTAGGCACCATCAATAGATCGGAAGAGC179570.18398443043155963No Hit
GAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC178650.18304181375841247No Hit
TAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT173090.17734513038591443No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA172210.17644349704638237No Hit
TCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGG171470.17568530537450314No Hit
TAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG165770.16984518033435228No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT162660.16665872614577876No Hit
AGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAAT159150.16306243862105427No Hit
TCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATA157530.16140261360964298No Hit
ACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCGG157460.16133089277581655No Hit
TAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATC153780.15756042608322793No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT152360.15610551773989212No Hit
AAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG146010.14959941352849598No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGCTGTAGGCACCATCAATAGATC139080.14249905097968096No Hit
CCTTGGTTCGATTCCGAGTCCGGGCCTGTAGGCACCATCAATAGATCGGA137020.14038840929850366No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGCTGTAGGCACCATCA136970.14033718013148477No Hit
TTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGAG132500.13575729259999805No Hit
TAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC128990.13216100507527356No Hit
TCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGA128410.1315667467378547No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCACTGTAGGCACC127160.13028601756238303No Hit
TGATCCCGTCATCACCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA124930.12800119671334156No Hit
AAAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG124920.12799095087993778No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCACCACTGTAGGCAC124860.12792947587951514No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCCTGTAGGCACCATC124390.12744792170953778No Hit
AACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATCAATAGATC123480.1265155508697944No Hit
AGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAGATCGGAAG117660.12055247582879827No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAG116590.11945617165459452No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATAGATCGGAA115160.11799101747785493No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCCTGTAGGCACCATCAA115130.11796027997764359No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCCTGTAGGCACCATC114970.11779634664318321No Hit
TTGGGTTCTACTCTGCTGGCAGGCTGTAGGCACCATCAATAGATCGGAAG114360.11717135080555303No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAAT113870.11666930496876814No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACTGTAGGCACCATC113680.11647463413409645No Hit
AAAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC111890.114640629954821No Hit
TCGATCCCGTCATCACCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC111770.11451767995397573No Hit
TCGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA109700.11239679243939461No Hit
ATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGA108560.11122876743136444No Hit
TAAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC107550.11019393825758333No Hit
TGATCCCGCATAGCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA107020.10965090908718333No Hit
CGATCCCGTCATCACCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA106740.10936402575187767No Hit
TTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAAT106480.10909763408337957No Hit
TAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCCTGTAGGCACCATC104390.10695625490199093No Hit
AGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC103910.10646445489860978No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC102710.10523495489015698Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCCTGTAGGCACCATCAA102230.10474315488677585No Hit
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT101960.10446651738487399Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAGATCGGAAG99340.10178210903308534No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCA98990.10142350486395325No Hit
TGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA98360.10077801735951554No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGC98220.1006345756918627No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)