FastQCFastQC Report
Wed 30 Aug 2023
SRR11915745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11915745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12609432
Total Bases630.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA150377511.925794912887431Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1054630.8363818449554271Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC950590.7538721807611953No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA854300.6775087093534428No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC845800.670767723716659No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT808010.6407980946326527No Hit
AAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG799120.6337478167137108No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA797020.6320823967328584No Hit
TCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG757610.6008280150921944No Hit
ACAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGA691020.5480183405564977No Hit
AAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT601650.4771428245142208No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC586610.46521524522278246Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA578180.45852977358536057No Hit
AAAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC551240.43716481440242516No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA545560.4326602498827862No Hit
AACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCG540560.42869496421408987No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA500460.3968933731511459No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA478400.379398532780858No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC470200.3728954642841962No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG465710.3693346377537069Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGAAG383310.30398672993359255No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG348070.2760393965406213Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC339360.26913186890575247No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA324310.2571963590429767No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCACTGTAGGCACC315420.25014608112403475No Hit
AGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCAATA312480.24781449315084136No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGTAGGCACC304920.24181898121977263No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT297680.23607724757150045No Hit
AACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATCAATAGATC285710.22658435368064161No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT275000.2180907117782942Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT274260.2175038494993272No Hit
TCGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC270010.21413335668093533No Hit
TCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAATAGATCGGAAGAGCA269340.21360200840133003No Hit
TTGGGTTCTACTCTGCTGGCCTGTAGGCACCATCAATAGATCGGAAGAGC268010.21254724241345685No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCTGTAGGCACCAT258950.20536214478177922No Hit
TAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCAAT249860.19815325543608941No Hit
TGGGGCTACAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATA246250.19529031918329073No Hit
TAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAA240420.19066679609359088No Hit
TAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT234670.18610671757459019No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT228370.18111045763203293No Hit
AAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG227580.1804839424963789No Hit
TCAAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA225520.17885024480087605No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCACCACTGTAGGCAC222660.1765821013983818No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCACTGTAGGCACC205380.16287807412736752No Hit
TTGGGTTCTACTCTGCTGGCAGGCTGTAGGCACCATCAATAGATCGGAAG204910.16250533727451005No Hit
AAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG201670.1599358321611949No Hit
TAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCA200400.15892864960134603No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAGATCGGA197330.15649396420076653No Hit
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT195290.15487612764793846Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGAGTCCCGTCCGTTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC193710.15362309737663046No Hit
ATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGA191900.1521876639645624No Hit
GAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC190630.15118048140471355No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA189330.15014950713085254No Hit
AGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCGGAA184040.1459542348933719No Hit
ACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCGG180350.14302785406987403No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCCTGTAGGCACCATC179640.14246478350491917No Hit
AAGTCCACTCAGGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC177970.14114037809157462No Hit
TTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGAG174280.1382139972680768No Hit
AGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAAT172160.1365327161445496No Hit
GAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAA170740.13540657501463985No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGCTGTAGGCACCATCAATAGATC168110.1333208347529056No Hit
AAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGA161310.1279280462434787No Hit
TAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCCTGTAGGCACCATC161000.12768219853201954No Hit
ACGAGGGTTCGATTCCCTTCGCCCGCCTGTAGGCACCATCAATAGATCGG157700.12506510999068No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGCTGTAGGCACCATCA157410.12483512342189561No Hit
TCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGA155400.12324107858307971No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACTGTAGGCACCATC154290.12236078516462914No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGC145380.11529464610301242No Hit
TCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGG143700.11396231011833048No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCCTGTAGGCACCATCAA142880.11331200326866427No Hit
AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCCTGTAGGCACCATCAA142540.11304236384319295No Hit
ACAGCTCAGCTGGGAGAGCGCTTGCCTGTAGGCACCATCAATAGATCGGA139460.11059974787127605No Hit
TAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG138250.10964014873945155No Hit
AGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAGATCGGAAG134850.10694375448473808No Hit
TCGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC134350.10654722591786846No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)