FastQCFastQC Report
Wed 30 Aug 2023
SRR11915746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11915746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5059808
Total Bases252.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3494836.90704074146687Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA511131.0101766707353323No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC430140.8501113085713925No Hit
AAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT342980.6778518078156325No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA323210.638779178972799No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA312200.6170194600269417No Hit
TCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC278540.5504951966556834No Hit
AAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG277790.5490129269727231No Hit
TCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG262210.5182212447586945No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA262060.5179247908221023No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT238860.47207324862919703No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT231240.4570133886503203No Hit
AAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGA220110.4350165065551894No Hit
AAGTCCACTCAGGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC216610.42809924803470806No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGTAGGCACC210970.41695258001884655No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC207670.4104305934138212Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC198060.3914377778761566No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG174210.34430160195801895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGAAG163760.3236486443754388No Hit
ACAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGA162800.32175133918124954No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCTGTAGGCACCAT146730.2899912407743535No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCACTGTAGGCACC138350.2734293475167437No Hit
AACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATCAATAGATC135490.26777695912572175No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA130410.25773705247313733No Hit
TAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT125810.24864579841764747No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC121150.2394359627875208No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAG120150.23745960321024037No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT119610.23639236903850896No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC118900.2349891537386399No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT116100.22945534692225475No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC114310.22591766327892285Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAA113160.22364484976505036No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCACTGTAGGCACC110550.21848655126834854No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA109050.21552201190242792No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACTGTAGGCACCATC108110.21366423389978434No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGC106780.21103567566200138No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT106260.21000796868181562Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAGATCGGA105270.20805137270030802No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCACCACTGTAGGCAC104060.20565997761179872No Hit
TGGGGCTACAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATA100350.19832768358008843No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC97020.1917464061877447No Hit
AACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCG96240.19020484571746596No Hit
TAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGA93560.18490820205035446No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA92320.18245751617452677No Hit
AGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAGATCGGAAG91950.18172626313093304No Hit
ACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACCATCAATAGATC91390.180619501767656No Hit
AGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCAATA87570.17306980818244486No Hit
AGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACCATCAAT86930.1718049380529854No Hit
GAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC86690.17133061175443812No Hit
TCGAGTCCCGTCCGTTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC83920.16585609572537138No Hit
AAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG82120.16229864848626668No Hit
AAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG80280.15866214686407074No Hit
AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCCTGTAGGCACCATCAA78430.155005881646102No Hit
AAGTCCCCCCCCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC75830.14986734674517294No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGCTGTAGGCACCATCAATAGATC71080.14047963875309102No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGCTGTAGGCACCATCA70170.13868115153776586No Hit
AGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACCATCAATAGATCGGA69580.13751509938717044No Hit
TCGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC68430.13524228587329795No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT66780.13198129257078528Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGAG66490.131408148293374No Hit
TCAAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA66160.13075594963287146No Hit
TGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT64900.12826573656549814No Hit
TAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTGTAGGCAC64370.12721826598953953No Hit
TCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGG63520.12553836034885119No Hit
AAAATCCTCTCGTGCCGACCACTGTAGGCACCATCAATAGATCGGAAGAG62670.12385845470816283No Hit
TCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGA61750.12204020389706488No Hit
AGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATCAATAGATCGGA61750.12204020389706488No Hit
TGATGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT61550.12164493198160879No Hit
AGGTAGAGCAACTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT60630.11982668117051082No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATAGATCG60590.1197476267874196No Hit
TAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAA60390.11935235487196352No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCCTGTAGGCACCATC60100.11877921059455221No Hit
ATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGA60040.11866062901991538No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCCTGTAGGCACCATCAA59710.11800843035941284No Hit
AAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC59080.1167633238257262No Hit
TAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCCTGTAGGCACCATC59060.11672379663418059No Hit
TCGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC59030.11666450584686217No Hit
AAAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG58520.11565656246244917No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAA58060.11474743705690019No Hit
TAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAA57370.1133837489485767No Hit
TAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC56110.1108935358812034No Hit
TAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCA56040.11075519071079376No Hit
AGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAAT54720.10814639606878364No Hit
TTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGAG54550.10781041494064597No Hit
AAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC54180.10707916189705222No Hit
AGGTCACCAGTTCGATTCCGGTAGTCGGCCTGTAGGCACCATCAATAGAT53930.10658507200273211No Hit
TCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAATAGATCGGAAGAGCA53630.105992164129548No Hit
TAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCATCA53310.10535972906481827No Hit
AAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATC53000.10474705759586135No Hit
AGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCATCAA52060.10288927959321775No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAAT51280.10134771912293905No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)