FastQCFastQC Report
Wed 30 Aug 2023
SRR11915747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11915747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16621487
Total Bases831 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10843766.523940968699129Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA2269271.3652629274384416No Hit
AAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1632920.9824151112352343No Hit
TCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1617110.9729033268804409No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA1543200.928436787875838No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT1509690.908276136786077No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA1232670.7416123479204959No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC1189120.7154113227053632No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA1168180.7028131718900962No Hit
AAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1147420.6903233146348459No Hit
AAAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1139050.6852876640940729No Hit
AACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCG1130950.6804144538933249No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC867830.5221133343845831No Hit
ACAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGA803150.483199848485277No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC765960.4608251957240649Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCAATA699980.42112958966908315No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC696380.4189637184687507No Hit
AGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGAAG673250.40504799600661484No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA582760.3506064168627031No Hit
GAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAA571750.3439824607750197No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA530570.31920730076677256No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAGATCGGA516000.310441538714316No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGTAGGCACC487740.2934394497917064No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT486800.292873916756064No Hit
TAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAA480190.2888971365798981No Hit
GAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC465190.27987267324517956No Hit
TAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCAAT445610.26809274043892706No Hit
AGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCGGAA427300.2570768788616807No Hit
AAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG423200.2546101922168576No Hit
TCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG420230.25282334847658333No Hit
AACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATCAATAGATC418900.2520231793942383No Hit
TCAAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA418850.25199309784978924No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGCTGTAGGCACCATCAATAGATC416190.25039275968509916No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA411770.24773355115580215No Hit
AGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAAT406220.2443944997219563No Hit
TAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT405620.24403352118856753No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG403280.24262570490835145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAATAGATCGGAAGAGCA401680.24166309548598147No Hit
TCGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC400660.24104943197922063No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCACTGTAGGCACC394710.2374697281897823No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC392050.23586939002509222Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGCTACAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATA384690.23144138668219033No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA375540.22593646404801207No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT373880.2249377567723032No Hit
AGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAGATCGGAAG359880.21651492432656597No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAA357360.21499881448633326No Hit
TCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGG348440.20963226695662066No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCCTGTAGGCACCATC346120.2082364832941842No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAG343750.20681061808729867No Hit
TAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCA342550.2060886610205212No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGCTGTAGGCACCATCA339920.20450637178250058No Hit
AAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG329690.19835168778822254No Hit
TAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCCTGTAGGCACCATC319920.19247375400287592No Hit
AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCCTGTAGGCACCATCAA318510.1916254544494124No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCACCACTGTAGGCAC312720.18814201160221106No Hit
ACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCGG301100.18115106067224912No Hit
AAAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG297890.17921982551861937No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACTGTAGGCACCATC285530.17178366773081133No Hit
AGGTCACCAGTTCGATTCCGGTAGTCGGCCTGTAGGCACCATCAATAGAT280200.16857697509254135No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCCTGTAGGCACCATCAA270800.16292164473611778No Hit
AAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGA269610.16220570397823011No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT259230.15596077535060493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATC257620.15499214961934513No Hit
CCTTGGTTCGATTCCGAGTCCGGGCCTGTAGGCACCATCAATAGATCGGA256530.15433637195035557No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCACTGTAGGCACC254130.1528924578168006No Hit
TGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA250150.15049796687865533No Hit
GCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAA244330.14699647510478456No Hit
ATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGA240630.14477044081555399No Hit
TCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC236350.1421954606107143No Hit
ACCAGTTCGATTCCGGTAGTCGGCCTGTAGGCACCATCAATAGATCGGAA232050.13960844778809503No Hit
TCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATA230030.13839315339235292No Hit
TAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG226180.13607687446977518No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT223250.13431409596506017Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGTCCACTCAGGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC222740.13400726421167974No Hit
AAGTCCCCCCCCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC216270.13011471235997116No Hit
TACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCCTGTAGGCACCATCA214290.1289234831997883No Hit
TAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCAAT210140.12642671501051622No Hit
ACGAGGGTTCGATTCCCTTCGCCCGCCTGTAGGCACCATCAATAGATCGG205820.12382766957011729No Hit
AAGAGGTCAGCGGTTCGATCCCGCTTAGCTCCCTGTAGGCACCATCAATA205570.12367726184787198No Hit
AAAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC204140.1228169296766288No Hit
AGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATCAATAGATCGGA200320.12051869968072049No Hit
TAAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC198320.11931543790275805No Hit
TGATCCCGCATAGCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA197610.11888827997158136No Hit
AGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCAATA196050.11794973578477064No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCCTGTAGGCACCATC194790.11719168086465429No Hit
ACAGCTCAGCTGGGAGAGCGCTTGCCTGTAGGCACCATCAATAGATCGGA193460.11639151178230923No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT191930.11547101652216796No Hit
TGATCCCGTCATCACCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA191140.1149957281198728No Hit
TGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGA190070.11435198306866286No Hit
AGCTCAGCTGGGAGAGCGCTTGCCTGTAGGCACCATCAATAGATCGGAAG189870.11423165689086663No Hit
TCGAGTCCCGTCCGTTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC184740.1111452904303929No Hit
ACCGAGCGGTCGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCA184630.11107911103260495No Hit
TCGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA184440.11096480116369854No Hit
TCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGA183390.11033308873026823No Hit
AACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAATAGATCGGA183380.11032707242137842No Hit
TAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC183240.11024284409692105No Hit
TTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGAG181980.1094847891768047No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGC178800.10757160294984439No Hit
AGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC177950.10706021669421033No Hit
TAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTGTAGGCAC177880.10701810253198164No Hit
TAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGTAGGCACCATCAAT177740.10693387420752429No Hit
ACAGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA176620.10626004761186529No Hit
TCGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAGATCGG176290.10606150941850148No Hit
TGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA175860.10580280813623955No Hit
TAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAA174850.10519516093836852No Hit
TCGATCCCGTCATCACCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC172300.10366100217146636No Hit
TAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCCTGTAGGCACCATCA167940.1010378914955082No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)