FastQCFastQC Report
Wed 30 Aug 2023
SRR11915748.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11915748.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17381575
Total Bases869 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8335674.795693140581334Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA2404371.383286612404227No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCA2042621.1751639307715211No Hit
TCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2042471.1750776324930279No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC1660340.9552298914223826No Hit
AAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1625700.9353007423090256No Hit
AAGTCCACTCAGGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1274130.733034837176723No Hit
ACAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGA1261990.7260504298373421No Hit
AAAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1177600.6774990183570822No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA1168920.6725052246416104No Hit
AAGTCCACTCAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1040010.5983404841045763No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT1037130.5966835571575073No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG924720.5320116272547223Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGTAGGCACC892490.5134690038158222No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT858220.4937527237894149No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCTGTAGGCACCAT741970.42687155795720466No Hit
TCGACGGGATTTGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG738560.42490971042612646No Hit
AGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGAAG695610.4001996366842476No Hit
TCGAGTCCCGTCCGTTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGC658600.3789069747706983No Hit
AAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGA647640.37260144722213034No Hit
TAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT634680.36514527596032004No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCACTGTAGGCACC628630.36166457872776203No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGC615400.354053070564664No Hit
ACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC596200.3430068909175377No Hit
AACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATCAATAGATC584870.3364884942820199No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC558570.3213575294528833Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGTCCCCCCCCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC551880.3175086262320877No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCACCACTGTAGGCAC512080.2946108163385654No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAGATCGGA484110.27851906400887144No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC480840.2766377615377203Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT473060.2721617574932076Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC467920.2692046031501748No Hit
TAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCAT458290.263664253670913No Hit
AAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG452280.2602065693126198No Hit
TGGGGCTACAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATA434130.2497644776149457No Hit
TAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCATCA402130.23135417820306847No Hit
AAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATC400430.23037613104681248No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA395400.22748226210800804No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCACTGTAGGCACC385930.22203396412580562No Hit
TCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCA367190.21125243253272502No Hit
TAGGGTGAGGTCCCCAGTTCGACTCTGGGTATCAGCCTGTAGGCACCATC361050.20771995633307108No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGCTGTAGGCACCATCA354920.20419323335198336No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT341610.19653569944035565No Hit
TAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCCTGTAGGCACCATC336120.19337718244750549No Hit
TAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCTCTGTAGGCACCATC334570.19248543356974268No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTGTAGGCAC334100.19221503229713074No Hit
GAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAA323410.1860648416498505No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACTGTAGGCACCATC321790.1851328202421242No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGCTGTAGGCACCATCAATAGATC319040.18355068513641598No Hit
AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCCTGTAGGCACCATCAA316950.18234826245607777No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCCTGTAGGCACC316430.18204909509063477No Hit
AGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCAATA316100.1818592388779498No Hit
ATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGA315670.18161185047960268No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT315210.18134720242555696No Hit
TAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATAGATCGGAAGA311960.17947740639153817No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC311050.17895386350201292No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCTGTAGGCACCATCAATAGA309920.178303749804031No Hit
TCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAATAGATCGGAAGAGCA308870.17769966185457878No Hit
TCAAATCCGGCCCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA308760.17763637645035044No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA300870.17309708700160947No Hit
TAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAA297500.17115825234479612No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAG296570.17062320301813846No Hit
AGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAGATCGGAAG289480.1665441710546944No Hit
TAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTGTAGGCAC279290.16068164133572477No Hit
AACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCAATAGATCG276640.1591570384156787No Hit
AGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCTGTAGGCACCATCAA275710.158621989089021No Hit
AGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATCAATAGATCGGA266260.153185197543951No Hit
ACGAGGGTTCGATTCCCTTCGCCCGCCTGTAGGCACCATCAATAGATCGG265110.15252357740883665No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA262680.15112554529724723No Hit
AGGTCACCAGTTCGATTCCGGTAGTCGGCCTGTAGGCACCATCAATAGAT257710.14826619566984006No Hit
TCGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATC252790.14543561213526393No Hit
AAAATCCTCTCGTGCCGACCACTGTAGGCACCATCAATAGATCGGAAGAG252790.14543561213526393No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA250450.1440893589907704No Hit
TAGGGTGAGGTCGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCA250250.14397429461944616No Hit
TCCTCTCTCGCCGACCACTGTAGGCACCATCAATAGATCGGAAGAGCACA243940.1403440137041666No Hit
AAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC241430.1388999558440475No Hit
TCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGG239280.137663013852312No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCCTGTAGGCACCA239010.13750767695102428No Hit
ACAGCTCAGCTGGGAGAGCGCTTGCCTGTAGGCACCATCAATAGATCGGA237020.13636278645634817No Hit
AAGTCCCGTCCTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG236180.1358795160967864No Hit
ACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACCATCAATAGATC235850.13568965988410142No Hit
ACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT232900.133992460407069Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCAGTTCGATTCCGGTAGTCGGCCTGTAGGCACCATCAATAGATCGGAA232300.13364726729309628No Hit
AACAGGGGTTCGAATCCCCTAGGGGACGCCTGTAGGCACCATCAATAGAT227390.13082243697708637No Hit
AAAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG225600.1297926108537345No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC224770.12931509371273892No Hit
GCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAA220830.12704832559765153No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCTGTAGGCACCATCAATAG216080.124315546778701No Hit
TAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAA215880.12420048240737677No Hit
TAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCAAT212060.12200275291508393No Hit
AGAGCGCACCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT210240.12095566713603342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATGACGAGGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT206560.11883848270366752No Hit
AGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATCAAT206340.11871191189521087No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATC204750.11779715014318323No Hit
ACGTGGGGGTTCAAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATA204550.117682085771859No Hit
ACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCCTGTAGGCACCATCAA204110.11742894415494567No Hit
ACGGACGTGGGGGTTCAAGTCCCCCCCCTCGCCTGTAGGCACCATCAATA203620.1171470364452013No Hit
TCGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCATC199570.11481698292588559No Hit
TAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCCTGTAGGCACCATCA197350.11353976840418661No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTGTAGGCACC194660.11199215260987569No Hit
TGAGTCTCGTTTCCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA193850.11152614190601254No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCTGTAGGCACCATC186740.10743560350543607No Hit
TCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCTGTAGGCACCA186280.10717095545139034No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCCTGTAGGCACCA181700.1045359813480654No Hit
AGCTCAGCTGGGAGAGCGCTTGCCTGTAGGCACCATCAATAGATCGGAAG180250.10370176465596473No Hit
AGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC178790.10286179474529782No Hit
AGAATACCTGCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT177500.10211962955025651No Hit
AAAATCCTCTCGTGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA176020.1012681532024572No Hit
TCGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAGATCGG175200.10079638928002785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)