FastQCFastQC Report
Wed 30 Aug 2023
SRR11915749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11915749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41304153
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA575133013.924338310484178Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA17309074.190636713940121Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG13913753.368607994455182Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG8372572.0270528244460064Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4138231.001891988924213Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT4020550.973400907167858Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA3576320.8658499788144791No Hit
TAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC3056310.7399522270799258No Hit
AAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2948210.7137805246847696No Hit
ACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2350300.5690226839901548Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2241780.5427492969048415No Hit
ACATCCTGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1517350.36736015383247295Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1477340.3576734765629984No Hit
ACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGCACC1424780.3449483639090723No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1252630.3032697462649821Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1127590.27299676136682915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAAAAGCCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1092390.2644746159060567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1028760.2490693853472797Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1007750.24398272977538119Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGAAAAGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG964830.23359152286696205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTA950450.23011003276111244No Hit
TCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA928890.2248902186663893No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG882170.21357900741845498No Hit
AAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT835830.20235979660447218Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG813520.1969584027058974No Hit
AAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT810040.19611587241602557No Hit
ACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGCA784200.1898598429073222No Hit
ACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG764850.18517508396794868Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA692180.16758121150674607Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGATGACGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA672500.16281655745367785Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACATCCTGGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT630800.15272072035952414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGACGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC620920.1503287090767846Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATCCTGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG614180.1486969119061708Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG600410.14536310670745387No Hit
TCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGA587840.1423198291948996No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC585270.14169761573370115No Hit
TAGGAAAAGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT546350.13227483444582439Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA520440.12600185748876147No Hit
AAATGAACCGTGAGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA466080.11284095330559134No Hit
TAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT448970.10869851271372154No Hit
TGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA440180.10657039741257979Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA434560.10520975941571784Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG427790.10357069905294995No Hit
TCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCACATCTC425090.10291701175908388TruSeq Adapter, Index 9 (97% over 38bp)
TAGGAAAAGCCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC421700.10209627104567427No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)