FastQCFastQC Report
Wed 30 Aug 2023
SRR11915750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11915750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30678414
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA29007089.455208473293307Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA8004122.609039698075657Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG6370882.076665371293314Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG5184061.689807041524376Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA4906401.5993004071201335No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2145610.6993875237487831Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2089560.6811173485043914Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2001200.6523153380745171No Hit
AAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1728720.5634971873057062No Hit
AGGAAAAGCCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1498240.48836944439174723Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1333110.4345433241757543No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG994200.32407151164985254No Hit
AGGAAAAGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG866280.2823744408690749Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAG864270.2817192570645927No Hit
TCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGA698920.22782142518840773No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC691770.22549079623216509No Hit
TAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC682080.22233222356279567No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG556290.18132945203751405Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG554240.18066122974936058No Hit
AAATGAACCGTGAGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA548510.1787934669634486No Hit
AGGAAAAGCCTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC501130.1633493830548085No Hit
ACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT495480.16150769723623914Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA492470.16052655133997473No Hit
TCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA472110.15388996315128936No Hit
GAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAA461960.15058144791970016No Hit
TGATGACGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA455290.14840728076751297Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGCACCATCAATAGA400630.13059019283069848No Hit
TAGGCTGAGGCGTGATGACGAGCTGTAGGCACCATCAATAGATCGGAAGA385680.12571705955855475No Hit
AAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT380160.12391774881191707No Hit
TTCTGCTGGCAGGTTGCTCCAGCCTGTAGGCACCATCAATAGATCGGAAG356630.11624786079228216No Hit
ACATCCTGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT355560.1158990813540752Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTAGGTGAAGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC337570.11003502332291362No Hit
TAGGCTGAGGCGTGATGACGCTGTAGGCACCATCAATAGATCGGAAGAGC323460.10543569820786694No Hit
TGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAA321910.10493045696560455No Hit
TAGAGCACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC320250.1043893598932461No Hit
TCTGCTGGCAGGTTGCTCCAGCCTGTAGGCACCATCAATAGATCGGAAGA315770.1029290497220619No Hit
TAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG311970.10169039377328959No Hit
AAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT309220.10079399802088856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)