FastQCFastQC Report
Wed 30 Aug 2023
SRR11915751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11915751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24678394
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA20904738.470863217436273Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG8735443.539711700850549Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA4201671.7025702726036387No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3515621.4245740626395704Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2409080.9761899416955577No Hit
AAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2278610.9233218336655132No Hit
ACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2059140.8343897905187834Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1884110.763465402165149Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1421650.5760707118947853Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA1318110.5341149833331943No Hit
TCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGA1152080.4668375097666404No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1148410.46535037895901976No Hit
TAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1025720.4156348261560294No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA961770.3897214705300515No Hit
AGGAAAAGCCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT884100.3582485959175463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT756850.30668527295576853No Hit
GAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAG696600.2822712045200348No Hit
TGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT687890.2787418014316491Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGG629090.25491529148938946No Hit
TCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA589070.23869867706950462No Hit
GAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAA561200.22740539761217848No Hit
AGAGCACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG554940.22486876577138692No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG524260.2124368384749834No Hit
TCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGCACCATCAATAGA506470.2052281035791875No Hit
ATGTAGGTGAAGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC419260.16988949929237698No Hit
ACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT404170.16377483883270524Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT381410.15455219654893265No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG376690.15263959234948593Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA353000.1430401022043817Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG346840.14054399163900214No Hit
TGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAA326790.13241947591889489No Hit
AGGAAAAGCCTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC300300.12168539006225446No Hit
TAACCGTGAGGCTTAACCTTACAACGCCCTGTAGGCACCATCAATAGATC292400.11848420930470596No Hit
AAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT289620.1173577178482522Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGAAAAGCCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC249180.10097091407163693No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)