FastQCFastQC Report
Wed 30 Aug 2023
SRR11915752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11915752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12378224
Total Bases618.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9171557.409423193504981Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3820443.0864201520347345Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1572861.2706669389728285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1420791.147814096755722No Hit
ACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1252711.0120272504359267Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1190800.9620119978439556Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC748750.6048929151710294No Hit
TAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC683020.5517915978899719No Hit
AGGAAAAGCCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT609150.49211421606201344Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC586480.4737997955118602Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA565510.4568587545353841No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC407900.3295303106487651No Hit
TCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGA397970.32150815819781575No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA327210.264343253119349No Hit
GAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAG319150.25783181819944445No Hit
TAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC315840.2551577673824613No Hit
AGAGCACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG307050.24805658711621312No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTA289600.23395924972758614No Hit
TGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT265440.21444110237462174Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG262990.2124618200478518No Hit
ACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT255900.2067340193552807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGCACCATCAATAGA244760.1977343437960082No Hit
AAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT227150.18350774715338808No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT205580.16608198397443769Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGTAGGTGAAGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC168670.13626348981889486No Hit
GAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAA168330.13598881390415943No Hit
AGGAAAAGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG167860.13560911484555457Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAAAAGCCTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC155030.12524413841597956No Hit
TCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA154600.12489675417087298No Hit
TAGGAAAAGCCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC149150.12049386083173161No Hit
TCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGG148220.11974254141789646No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC145280.1173674026257725Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAA140140.11321494909124281No Hit
ATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA129460.1045868938872006Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)