FastQCFastQC Report
Wed 30 Aug 2023
SRR11915753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11915753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17780946
Total Bases889 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA13875047.8033193509501695Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG3744722.106029679185798Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2271931.2777329170225251No Hit
ACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2240631.2601298041172837Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1412370.7943165678586505No Hit
TACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG987640.5554485121320316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT985750.5543855765604372Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC931620.5239428768300629Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG895890.5038483329289679No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC804180.4522706497168374No Hit
AGAGCACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG656380.3691479632186049No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA628610.35353012151321983No Hit
TCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGCACCATCAATAGA610840.3435362775411387No Hit
ACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT547410.3078632599187917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC504510.28373630964291774No Hit
TAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC482600.2714141306092488No Hit
ACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT454180.25543072905119896No Hit
ACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGCACC425650.2393854635180828No Hit
AAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT356820.2006754871197517No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA349460.19653622478803998No Hit
TCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGA346680.19497275341818146No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTA340990.1917726987079315No Hit
AGGAAAAGCCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT308160.17330911414949463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAG289580.1628597263610159No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA287210.16152683889822286Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGGGGGTTCGAATCCCCCCCTCACCGCCACTGTAGGCACCATCAATAG264950.14900781994388823No Hit
ATGTAGGTGAAGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC253720.1426920704893879No Hit
AAATGAACCGTGAGGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA253530.14258521453245512No Hit
TACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG237310.13346309020903613No Hit
TATCCGGGGTTCGAATCCCCGCCTCACCGCCACTGTAGGCACCATCAATA229020.12880079608812717No Hit
AAGTCCCCCCCCTCGCACCACTGTAGGCACCATCAATAGATCGGAAGAGC206650.11621991315872619No Hit
TAGGCTGAGGCGTGATGACGAGCTGTAGGCACCATCAATAGATCGGAAGA202840.11407717002233739No Hit
CGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAA202520.11389720209487167No Hit
AAATGAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAG190520.10714840481490694No Hit
AAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT181580.10212055084133319Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)