FastQCFastQC Report
Wed 30 Aug 2023
SRR11915754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11915754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23717958
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8351223.5210535409498576Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG4148491.749092396571408Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT2729691.1508958739196689Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2444661.0307211101394143No Hit
ACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2238250.9436942252785843No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA1584310.6679790899368319No Hit
ACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGCACC1529570.6448995314014807No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1384250.5836295013255357Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1255870.529501738724725Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTA1065830.4493767971087562No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAA1052460.44373971823375347No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC938160.39554838574214524No Hit
TACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG862520.3636569387634467Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC862470.3636358576906157No Hit
ACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGCA784980.33096441101717106No Hit
TACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG781720.32958992506859147No Hit
ATGTAGGTGAAGCGACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC640380.2699979483899921No Hit
TTGGTTCTCCCCGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACG637970.2689818406795391No Hit
TCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGA561500.23674044789184634No Hit
AGAGCACTGTTTCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG541260.22820682960986777No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC515340.21727840145429048Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT512460.21606413165922633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT477780.20144229954366225No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA459690.1938151673934156No Hit
ATGTACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC456230.19235635715351213No Hit
TAAACGGCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC422590.1781730113528323No Hit
TGGTTCTCCCCGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT384750.16221885543435063No Hit
TAACCGTGAGGCTTAACCTGTAGGCACCATCAATAGATCGGAAGAGCACA370190.15608004702597078No Hit
ATGTACACGGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC322510.1359771359743533No Hit
ATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA300860.1268490314385412Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTACACGGAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT292130.123168276122253No Hit
TCTGGTTCTCCCCGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC291710.12299119511047284No Hit
GTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC291510.12290687081914894No Hit
AACGCGATGTACACGGAGTCGACCCTGTAGGCACCATCAATAGATCGGAA287740.12131735792769345No Hit
TACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCGACCCTGTAGGC285210.12025065564244611No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAAT275480.11614827886953843No Hit
TAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC270540.11406546887383813No Hit
ACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG260540.10984925430764318Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGATGACGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT241140.10166979804922499No Hit
TAAACGGCGGCCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT239530.1009909875040676No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)