FastQCFastQC Report
Wed 30 Aug 2023
SRR11917594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11917594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27298428
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA408328414.957945563751876Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA375686913.762217370172378No Hit
ATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATA15244915.584537688397296No Hit
TCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAG10046773.680347454439501No Hit
TCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGA5442061.9935433644750533No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG5307501.9442511488207308No Hit
TCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAA5053001.851022337256929No Hit
GTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGG3141811.1509124261660781No Hit
TGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATC3088651.1314387773537729No Hit
ACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAG2655730.9728508909011171No Hit
CATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAAT2621450.960293391253152No Hit
TTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGA2044760.7490394684997979No Hit
ACAACCAGGAGGTTGGCTTAGAAGCAGCCACCCTTGCTGTAGGCACCATC2013210.7374820264375662No Hit
ATCCTGTCAGCCCGACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1953000.7154258113324328No Hit
TACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGA1680820.615720436356262No Hit
TCTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATC1607170.5887408608290557No Hit
TTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGAT1486120.544397648099004No Hit
TAGGGAACTGGTGCTAACCGGCCGAAAACTTACCTGTAGGCACCATCAAT1393760.5105641980556537No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1346730.4933360997929991Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGA1333960.4886581747491101No Hit
AAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATAG1232010.4513117019045932No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTACCTGTAGGCACCATCAA1176800.43108709409933793No Hit
TGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCATCAA1163910.4263652104802518No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATGCTGTAGGCACCATCAA1118380.4096865944075607No Hit
CGGGCTGTGGCGCAGTTTGGTAGCGCACTTGACCTGTAGGCACCATCAAT982960.36007934229765903No Hit
CGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAA922470.3379205571837323No Hit
TTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA863940.31647976213135787No Hit
TGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAA746160.27333442057542656No Hit
TAAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATA718990.2633814665078883No Hit
AGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATAGA658830.24134356747575356No Hit
TGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCG648810.23767302644679758No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC617140.22607162580936896Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATAGGTGGGAGACTGTGAAACCTCGACGCTGTAGGCACCATCAATAGATC610540.2236539041735297No Hit
GGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCG603860.2212068768208924No Hit
GCGAATTCGGTGATCCTCTGCTGCCAAGAAAAGCCTCTCTGTAGGCACCA582320.21331631257301703No Hit
TTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCATCA580550.21266792358886014No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC577460.2115359902775354Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAA569490.20861640824152952No Hit
TGTGTGCGGGGTTGTGGGAGGATATGCTGTAGGCACCATCAATAGATCGG484820.17759997022539173No Hit
GCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA482120.1766109022834575No Hit
TCCCGCATCCTCCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA468760.1717168475781829No Hit
CTTATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA466350.1708340128596416No Hit
AATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG453940.16628796354134384No Hit
TTGTGTGTGCGGGGTTGTGGGAGGATATGCTGTAGGCACCATCAATAGAT445820.16331343328634162No Hit
TTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCAT445160.1630716611227577No Hit
CGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC391280.14333426085926998No Hit
TAGGGAACTGGTGCTAACCGGCCGAAAACTTACACTGTAGGCACCATCAA385850.14134513533160226No Hit
AATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGA380020.13920948121994425No Hit
TCCCTCCGCCACCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA376010.1377405321654419No Hit
ATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCTGTAGGCAC365560.13391247290869643No Hit
CTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTAGGCACCATCAATAG358540.1313408962596674No Hit
TAGGGAACTGGTGCTAACCGGCCGAAAACTTCTGTAGGCACCATCAATAG349750.1281209306264815No Hit
AAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCTGTAGGC341450.12508046250868365No Hit
CTCATCTTGGTCGTACGCGGAATAGTTTAAACTGCTGTAGGCACCATCAA327260.11988236099162927No Hit
AATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCTGTAGGCA326520.11961128311124729No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTAAACTCCAGTCA324910.1190215055606865Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTACACTGTAGGCACCATCA323370.11845737051232401No Hit
GAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC310770.11384172011663089No Hit
GCCGCCTTAGCTCAGTCGGTAGAGCGATTCACCTGTAGGCACCATCAATA304240.11144964098298994No Hit
TTCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATA301810.11055947983524912No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTCTGTAGGCACCATCAATA295690.10831759250019818No Hit
CCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAA294360.10783038495843057No Hit
CTCATTTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA282520.10349313887231895No Hit
TATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGA280020.10257733522237983No Hit
AGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT277420.10162489942644316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATC274560.10057722005091282No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)