FastQCFastQC Report
Wed 30 Aug 2023
SRR11917595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11917595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27474303
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG25594729.315876002386666No Hit
TAATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGG16331025.944107117112306No Hit
ATAGGTGGGAGACTGTGAAACCTCGACGCTGTAGGCACCATCAATAGATC6919042.5183677999037863No Hit
TTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCATCA5465671.989375308265327No Hit
TTGTGTGCGGGGTTGTGGGAGGATATGCTGTAGGCACCATCAATAGATCG4233241.540799779342901No Hit
AGGTGGGAGACTGTGAAACCTCGACGCTGTAGGCACCATCAATAGATCGG3214301.1699295883866463No Hit
AATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGA2974491.0826443895592184No Hit
CTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA2827181.0290270148072547No Hit
ATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATA2599010.9459785021661877No Hit
TATAGGTGGGAGACTGTGAAACCTCGACGCTGTAGGCACCATCAATAGAT2549280.9278779519902653No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTACCTGTAGGCACCATCAA2533610.9221744406036434No Hit
CCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAA1971320.7175141076372347No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1805790.6572650814835957Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCG1784600.6495524199467407No Hit
TCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAG1746150.6355575244256424No Hit
TCTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATC1540520.5607130415646941No Hit
TCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGA1473930.5364758479951248No Hit
TATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGA1451200.5282026626844728No Hit
TATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG1017530.3703569841244016No Hit
TCCCGCATCCTCCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA938380.3415482460100989No Hit
TAAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATA890930.324277562200577No Hit
ACAACCAGGAGGTTGGCTTAGAAGCAGCCACCCTTGCTGTAGGCACCATC887500.32302912288621116No Hit
ACCAAATAAGCGCCGATTCACATCAGCTGTAGGCACCATCAATAGATCGG868540.3161281288919322No Hit
TTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCTGTAGGCACCATCAAT866040.3152181877007035No Hit
ACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAG840750.3060132226102333No Hit
AAATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGG800490.29135952966668527No Hit
TTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGA786290.2861910637005059No Hit
GTCCCCTTAGCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC755990.2751625764628133No Hit
ATCCTGTCAGCCCGACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC754340.2745620152766023No Hit
TTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGAT718480.26150981882961694No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTACACTGTAGGCACCATCA695570.2531711177531965No Hit
TAGGTGGGAGACTGTGAAACCTCGACGCTGTAGGCACCATCAATAGATCG680310.24761683672193613No Hit
TGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATC668770.24341654818322414No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG653570.23788410574055327No Hit
TCCCTCCGCCACCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA627730.2284789535880128No Hit
TTGTGTGCGGGGTTGTGGGAGGCTGTAGGCACCATCAATAGATCGGAAGA615250.22393652716139878No Hit
TTTAGGGAACTGGTGCTAACCGGCCGAAAACTTACCTGTAGGCACCATCA612320.22287007608527867No Hit
TGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCG599580.21823301577477688No Hit
TCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAA574430.20907900739101554No Hit
TACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAG533270.19409773561862514No Hit
TAATCCTGAGAGGTGACGCATAGCCTGTAGGCACCATCAATAGATCGGAA478520.17417002353071523No Hit
ACGGAGGCGCCCAAAGGTTCCCTCAACCTGGACGCTGTAGGCACCATCAA457920.16667210811499023No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTCTGTAGGCACCATCAATA455150.16566389327510875No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTACAACCTGTAGGCACCAT450130.16383673136312138No Hit
TGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGA431220.156953936192667No Hit
CAGTGTGGGGGTCAGGGGTTCGAGTCCCCTTAGCTCCACCACTGTAGGCA389010.14159048911996056No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGC382370.13917368531605698No Hit
AGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATAGA380210.13838749612683532No Hit
TGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCATCAA350390.12753371759785861No Hit
TGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCATC349720.12728985335860932No Hit
AAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATAG348940.12700595170694592No Hit
ACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA333010.12120780643643625No Hit
AGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT313490.11410298561532208Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCTGAACGGAAGCCCCAGTAAACGGCGGCCGTACTGTAGGCACCATC310560.11303653453920195No Hit
TACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGA309460.1126361604150613No Hit
AATAGGTGGGAGACTGTGAAACCTCGACGCTGTAGGCACCATCAATAGAT308350.11223214652615572No Hit
CCTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATC308350.11223214652615572No Hit
CTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCATCA307990.11210111499461879No Hit
TCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT299230.10891268106055321No Hit
TTAGGTGGGAGACTGTGAAACCTCGACGCTGTAGGCACCATCAATAGATC294920.10734394244687481No Hit
ATGTCTCAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG291430.10607366454391945Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGTGCGGGGTTGTGGGAGGATATGCTGTAGGCACCATCAATAGATCGG286820.10439573298729361No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTACTGTAGGCACCATCAAT286680.1043447762805848No Hit
TAGGGTGAAGCGGAGAATTTAAGCCCCAGCTGTAGGCACCATCAATAGAT280180.10197892918339002No Hit
TGGGCGAGTGGCGGAATGGCAGACGCGCTGGCCTGTAGGCACCATCAATA277800.10111266516934024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)