FastQCFastQC Report
Wed 30 Aug 2023
SRR11917596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11917596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27822406
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG293002010.531152481924101No Hit
CTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA11307324.064105742688105No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8462033.041444366817162Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATA6037232.169916577308231No Hit
AATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGA4997461.7961997966674772No Hit
TATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGA4589361.649519455650241No Hit
TCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGA3456761.242437480065527No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTACCTGTAGGCACCATCAA2933221.054265400339568No Hit
AAATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGG2793931.0042014339090588No Hit
TCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAG2434170.8748955787648273No Hit
AGGTGGGAGACTGTGAAACCTCGACGCTGTAGGCACCATCAATAGATCGG2378920.8550374830990533No Hit
TAATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGG2285800.8215680556167572No Hit
ATAGGTGGGAGACTGTGAAACCTCGACGCTGTAGGCACCATCAATAGATC2250150.8087546418523257No Hit
ACCAAATAAGCGCCGATTCACATCAGCTGTAGGCACCATCAATAGATCGG2234060.8029715330873972No Hit
ACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAG1750770.6292662108374093No Hit
TAAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATA1700240.6111045895886934No Hit
TCTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATC1567170.5632762313942223No Hit
TTGTGTGCGGGGTTGTGGGAGGCTGTAGGCACCATCAATAGATCGGAAGA1494740.5372432563883943No Hit
TTGTGTGCGGGGTTGTGGGAGGATATGCTGTAGGCACCATCAATAGATCG1427360.5130253652397999No Hit
TCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAA1276810.4589143009414786No Hit
AGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATAGA1228750.4416404533813503No Hit
AAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATAG1201060.4316880430829742No Hit
TCCCGCATCCTCCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA1093140.3928991619200726No Hit
GTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGG1062400.3818505128564366No Hit
ACAACCAGGAGGTTGGCTTAGAAGCAGCCACCCTTGCTGTAGGCACCATC1046510.37613928860070545No Hit
TACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAG928520.33373102239971625No Hit
TTTGTGTGTGCGGGGTTGTGGGAGGACTGTAGGCACCATCAATAGATCGG820920.29505715645153047No Hit
TCCCTCCGCCACCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA793760.28529524010252744No Hit
ACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA783670.28166866661352No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG759970.27315035227363155No Hit
TTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCATCA756090.2717557927952025No Hit
TACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGA753990.27100100544863015No Hit
TGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGA742480.2668640519443214No Hit
TATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCG723560.26006377737425007No Hit
TGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATC723400.2600062697668922No Hit
TATAGGTGGGAGACTGTGAAACCTCGACGCTGTAGGCACCATCAATAGAT701010.2519587989622465No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTCTGTAGGCACCATCAATA688950.24762416305764498No Hit
TGTGTGCGGGGTTGTGGGAGGATATGCTGTAGGCACCATCAATAGATCGG666240.23946167703828347No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTACACTGTAGGCACCATCA642940.23108713171678968No Hit
TTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGA636780.22887308883351065No Hit
CATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAAT635520.22842021642556723No Hit
CCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAA598800.21522222053692985No Hit
TGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCG575070.20669312352066174No Hit
TAGGGAACTGGTGCTAACCGGCCGAAAACTTACCTGTAGGCACCATCAAT538550.1935670121412217No Hit
CGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAA492650.177069517280425No Hit
TTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGAT470210.16900407534847994No Hit
CATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGA458150.16466943944387843No Hit
TGTGTGCGGGGTTGTGGGAGGCTGTAGGCACCATCAATAGATCGGAAGAG451850.16240507740416124No Hit
AGGGTGAAGCGGAGAATTTAAGCCCCAGCTGTAGGCACCATCAATAGATC428170.15389395151519247No Hit
CCTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATC401310.1442398619299855No Hit
ATGTACACGGAGTCGCCCCAACGCGACTGTAGGCACCATCAATAGATCGG392280.1409942763397242No Hit
CTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGA389140.13986568954532544No Hit
TCCTGCACCTGCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCACA384970.13836689752856024No Hit
CCCTCGGCCCGGACCCTGGCATCAGCTAGAGGGCTGTAGGCACCATCAAT367460.13207340874833037No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTCTGTAGGCACCATCAATAG364880.1311460985796843No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTACTGTAGGCACCATCAAT359850.12933820317337041No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGC358790.12895721527462434RNA PCR Primer, Index 20 (100% over 50bp)
TGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCATCAA349130.1254851934803913No Hit
TTTGTGTGTGCGGGGTTGTGGGAGGCTGTAGGCACCATCAATAGATCGGA346250.12445005654794915No Hit
AAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC341880.1228793800219866No Hit
AGCTCTGAACGGAAGCCCCAGTAAACGGCGGCCGTACTGTAGGCACCATC329700.11850161341186669No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC329520.11843691735358905Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCCCCTTAGCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC328840.118192510022318No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGC326720.11743053422482584No Hit
TTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAAT326670.11741256309752651No Hit
TAGGGTGAAGCGGAGAATTTAAGCCCCAGCTGTAGGCACCATCAATAGAT318920.11462703836612838No Hit
ATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGAA312890.11245972041382761No Hit
CAGTGTGGGGGTCAGGGGTTCGAGTCCCCTTAGCTCCACCACTGTAGGCA301160.1082436939494018No Hit
GCGGGCGTAGCTCAATGGTAGAGCCCTAGTCCTGTAGGCACCATCAATAG300210.10790224253071429No Hit
GAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC295590.106241710368255No Hit
ATCCTGTCAGCCCGACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC294580.10587869359680827No Hit
TGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAA290250.10432239397268517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)