FastQCFastQC Report
Wed 30 Aug 2023
SRR11917597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11917597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19101734
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA424549522.225704744920016Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA11710606.130647615551552No Hit
ATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATA4820612.5236504706850176No Hit
TCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAG3808951.9940336306641062No Hit
ATCCTGTCAGCCCGACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3109501.6278626851363338No Hit
TCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGA2797151.4643434988676944No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2177601.1400012166434734Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGG1788560.9363338427809749No Hit
TCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAA1537650.8049792757034518No Hit
ACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAG1463640.7662341020977468No Hit
ACAACCAGGAGGTTGGCTTAGAAGCAGCCACCCTTGCTGTAGGCACCATC1365730.7149769753887264No Hit
TGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATC1194460.6253149583173967No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1070020.5601690401510145No Hit
TGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCATCAA1062500.5562322247812685No Hit
AAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATAG1019420.5336792984343726No Hit
CATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAAT937790.49094495819070666No Hit
CGGGCTGTGGCGCAGTTTGGTAGCGCACTTGACCTGTAGGCACCATCAAT905390.4739831472891414No Hit
TATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGA881520.46148689956629063No Hit
TGTGTGCGGGGTTGTGGGAGGATATGCTGTAGGCACCATCAATAGATCGG784710.41080563680763227No Hit
TAGGGAACTGGTGCTAACCGGCCGAAAACTTACCTGTAGGCACCATCAAT781080.4089052857714383No Hit
TACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGA740120.3874622063106941No Hit
TTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGA731770.383090875414766No Hit
AATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGA694630.3636476143998236No Hit
TCCCGCATCCTCCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA634450.33214262118821253No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC591170.30948499230488713Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATAGA589050.3083751454187353No Hit
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATGCTGTAGGCACCATCAA584110.30578899276892874No Hit
TTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGAT579100.30316619423137187No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTACCTGTAGGCACCATCAA561590.29399948716697655No Hit
TGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAA547300.28651849093909487No Hit
CGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAA539990.2826916132325997No Hit
CTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGA536590.28091167011329965No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC536320.2807703216891199Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATA496120.2597251118668075No Hit
ATAGGTGGGAGACTGTGAAACCTCGACGCTGTAGGCACCATCAATAGATC472080.24713986698799176No Hit
TGTGTGCGGGGTTGTGGGAGGCTGTAGGCACCATCAATAGATCGGAAGAG454210.2377846953580235No Hit
TCCCTCCGCCACCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA441600.23118320043614887No Hit
TTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCAT441260.23100520612421888No Hit
TTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCATCA432830.22659199421371903No Hit
TCTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATC421010.22040407431074058No Hit
ATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGAA368200.19275736956655348No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCAGAACTCCAGTCA362780.18991993082931635Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCGAATTCGGTGATCCTCTGCTGCCAAGAAAAGCCTCTCTGTAGGCACCA350710.18360113275580112No Hit
TGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCG346400.18134479309574722No Hit
TTGTGTGCGGGGTTGTGGGAGGATATGCTGTAGGCACCATCAATAGATCG338750.17733992107732208No Hit
AATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCTGTAGGCA331470.17352874875129137No Hit
CTGTAGGCACCATCAATAGATAGGAAGAGCACACGTCTGAACTCCAGTCA315030.16492220025679344Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCTGTAGGCAC313370.1640531692044293No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCAAGTCA310910.1627653280063475Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCTGTAGGC307030.16073409879961684No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCAAACGTCTGAACTCCAGTCA294620.1542373064141716Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTGTGTGTGCGGGGTTGTGGGAGGATATGCTGTAGGCACCATCAATAGAT294200.15401743108766983No Hit
GTCAAGTGGTCGCAGGTTCAAATCCTGTCAGCCCGACCACTGTAGGCACC292050.15289187882105362No Hit
AGGGTGAAGCGGAGAATTTAAGCCCCAGTAAACGGCGGTGGTCTGTAGGC286250.14985550526460056No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGACA275460.14420680342423364Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACAAGTCTGAACTCCAGTCA275070.14400263347819625Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG273910.14339535876690565No Hit
TTGTGTGTGCGGGGTTGTGGGAGGACTGTAGGCACCATCAATAGATCGGA273220.14303413501622417No Hit
TTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA272170.14248444669996976No Hit
TTCTCCTAGGCGGCTCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC269040.14084585200484942No Hit
GGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCG269010.140830146624385No Hit
GCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA267620.14010246399620055No Hit
GCCGCCTTAGCTCAGTCGGTAGAGCGATTCACCTGTAGGCACCATCAATA256040.1340401871369374No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTACAGTCA237430.12429761612218033Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TCCTGCACCTGCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCACA236840.12398874363971354No Hit
AGGGTGAAGCGGAGAATTTAAGCCCCAGTAAACGGCGGTGGCTGTAGGCA235300.12318253410920706No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGAACACGTCTGAACTCCAGTCA217150.11368077892823761Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TAGGGAACTGGTGCTAACCGGCCGAAAACTTACACTGTAGGCACCATCAA214130.11209977062815345No Hit
CCTAAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAA205960.10782267201501185No Hit
CGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC204940.10728868907922182No Hit
TGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCTGTAGGCACCATCAATA203160.10635683650500002No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGACTGAACTCCAGTCA199330.10435178293237671Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC198370.10384921075751552No Hit
TTTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCA195300.10224202682332399No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)