FastQCFastQC Report
Wed 30 Aug 2023
SRR11917598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR11917598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17570234
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA313974017.869653870289945Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA198098011.27463641064769No Hit
ATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATA5290113.01083639523526No Hit
TCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAG4344242.4724997971000273No Hit
TCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGA3832902.181473507979461No Hit
GTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGG2611201.486149814510154No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG2429621.3828045773323223No Hit
TCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAA1721750.9799243424987966No Hit
ACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAG1539290.8760782582633788No Hit
ACAACCAGGAGGTTGGCTTAGAAGCAGCCACCCTTGCTGTAGGCACCATC1493990.8502960176853649No Hit
AATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGA1434340.8163465552023951No Hit
AAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATAG1423230.8100233611003701No Hit
TGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATC1281000.7290739554180098No Hit
CGGGCTGTGGCGCAGTTTGGTAGCGCACTTGACCTGTAGGCACCATCAAT1197020.6812772100815504No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1126100.6409134903951763Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAA1111930.6328487144792722No Hit
CATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAAT1094510.622934219316601No Hit
TGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAA1041380.5926955782148376No Hit
TACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGA1040790.5923597830285016No Hit
TAGGGAACTGGTGCTAACCGGCCGAAAACTTACCTGTAGGCACCATCAAT971510.5529294601312652No Hit
ATCCTGTCAGCCCGACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC916020.5213476382841572No Hit
CTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGA765790.43584507753283197No Hit
TGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCATCAA721350.41055230112473173No Hit
TTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGA662870.3772687375705981No Hit
TGTGTGCGGGGTTGTGGGAGGATATGCTGTAGGCACCATCAATAGATCGG589190.33533417938543103No Hit
TTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGAT572620.32590345694883743No Hit
GGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCG507090.2886074254901784No Hit
TCTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATC504550.28716179875578207No Hit
TTAGGGAACTGGTGCTAACCGGCCGAAAACTTACCTGTAGGCACCATCAA501450.2853974511665582No Hit
TATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGA491190.2795580297906106No Hit
AGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATAGA471670.2684483314223362No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC467680.26617744533168997Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCCCTTAGCTCAGTCGGCAGAGCGTTTCCATGCTGTAGGCACCATCAA458700.2610665287667768No Hit
GGCAGGTTGCCCGAGCGGCCAATGGGAGCGGACCTGTAGGCACCATCAAT429220.24428815233764103No Hit
GCGAATTCGGTGATCCTCTGCTGCCAAGAAAAGCCTCTCTGTAGGCACCA425480.24215955234289993No Hit
ACCAAATAAGCGCCGATTCACATCAGCTGTAGGCACCATCAATAGATCGG422370.24038951330983982No Hit
AAATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCTGTAGGC394090.22429411014104877No Hit
TGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCG372540.21202904867402447No Hit
TTGTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCAT364160.20725961873928372No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC361290.20562617435829256Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCTGTAGGCA360930.20542128238018914No Hit
TTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA357510.2034748085882066No Hit
ATAAGCGCCGATTCACATCAGCGCGACTACGCTCTCGCTGCTGTAGGCAC356840.2030934818511808No Hit
ATCCTGAGAGGTGACGCATAGCCGCTGTAGGCACCATCAATAGATCGGAA350830.19967292410562087No Hit
TAAGCGACGGCTAGTCTGTCAGACCGGGAACGCTGTAGGCACCATCAATA330300.18798838990988964No Hit
TTGTGTGTGCGGGGTTGTGGGAGGACTGTAGGCACCATCAATAGATCGGA282240.1606353108330828No Hit
TCCCGCATCCTCCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA267040.15198431620204944No Hit
TCCCTCCGCCACCGCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA263800.15014028839911864No Hit
TTGTGTGTGCGGGGTTGTGGGAGGATATGCTGTAGGCACCATCAATAGAT251180.14295768627782648No Hit
GCGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCA236920.13484168736739646No Hit
ATAGGTGGGAGACTGTGAAACCTCGACGCTGTAGGCACCATCAATAGATC236350.1345172750687327No Hit
CTTATCTTGGTCGTACGCGGAATAGTTTAAACTGTCTGTAGGCACCATCA229710.13073815636149183No Hit
TAGGGAACTGGTGCTAACCGGCCGAAAACTTACACTGTAGGCACCATCAA224940.1280233376516215No Hit
TAGGGAACTGGTGCTAACCGGCCGAAAACTTCTGTAGGCACCATCAATAG222330.12653787081037166No Hit
GCCGCCTTAGCTCAGTCGGTAGAGCGATTCACCTGTAGGCACCATCAATA218660.12444911092248402No Hit
TTGTGTGCGGGGTTGTGGGAGGATATGTCTCAGCGCTGTAGGCACCATCA211970.1206415349960621No Hit
CTCATCTTGGTCGTACGCGGAATAGTTTAAACTGCTGTAGGCACCATCAA200070.11386871683097675No Hit
CGGAATAGTTTAAACTGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC197140.11220112378696834No Hit
CTGTAGGCACCATCAATAGATAGGAAGAGCACACGTCTGAACTCCAGTCA193250.10998715213468414Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTCATCTTGGTCGTACGCGGAATAGTTTAAACTGTAGGCACCATCAATAG189100.1076252029426586No Hit
TGTGTGCGGGGTTGTGGGAGGCTGTAGGCACCATCAATAGATCGGAAGAG185690.10568442059451229No Hit
TTGTGTGCGGGGTTGTGGGAGGATATGCTGTAGGCACCATCAATAGATCG178050.10133615750365077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)