FastQCFastQC Report
Mon 25 Sept 2023
SRR1200730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1200730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10558976
Total Bases300.8 Mbp
Sequences flagged as poor quality0
Sequence length24-49
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGGCGTGATGACGAGGCAC226712021.47102143238132No Hit
AGGCTGAGGCGTGATGACGAGGCA5111134.84055461438685No Hit
GGCTGAGGCGTGATGACGAGGCAC2383192.257027575401251No Hit
AAGGCTGAGGCGTGATGACGAGGCAC1668081.57977440236629No Hit
CTCATCTCCGGGGGTAGAGCACTGTTTCGGC1254061.1876719863744363No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCC415720.39371242059836103No Hit
AAGGCTGAGGCGTGATGACGAGGCA373630.35385060066430685No Hit
GGCACGCTGATATGTAGGTGAAGCGAC348270.3298331201813509No Hit
AGGCTGAGGCGTGATGACGAGGCACT340290.32227556914609906No Hit
TCTCCGGGGGTAGAGCACTGTTTCGGC304470.288351825025457No Hit
GCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGT275170.2606029221015371No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGC242290.2294635388886195No Hit
GGTGGTTCAAGTCCACTCAGGCCT199650.1890808351112835No Hit
GGCTTGTAGCTCAGGTGGTTAGAGCGCACCCC194430.18413717390777287No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGC193350.18311434745187413No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCAC166570.157752039591718No Hit
CGATGTCGACTTGGAGGTTGTGCC156570.14828142425932211No Hit
AAGGCTGAGGCGTGATGACGAGGC150240.14228652475391554No Hit
GAGCGGTAGTTCAGTCGGTTAGAATACCTGCC149980.14204028875527325No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCA135340.12817530790864567No Hit
CGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGC133170.1261201843815158No Hit
CCGGGGGTAGAGCACTGTTTCGGC124560.11796598458032294No Hit
CGGTGCTGAAGCAACAAATGCCCTGCTTCC123490.11695262873975658No Hit
CAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCT121660.11521950613392815No Hit
TCATCTCCGGGGGTAGAGCACTGTTTCGGC119350.11303179399214469No Hit
GGGCTACAGCTCAGCTGGGAGAGCGCCTGC117780.11154490738495854No Hit
AGGCACGCTGATATGTAGGTGAAGCGAC114280.10823019201862No Hit
CATCTCCGGGGGTAGAGCACTGTTTCGGC113050.10706530633273528No Hit
AACCGGGAGATAGCTGGTTCTCCCCGAAAGCT106980.101316642825971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)