FastQCFastQC Report
Mon 25 Sept 2023
SRR1200731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1200731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8589645
Total Bases253.5 Mbp
Sequences flagged as poor quality0
Sequence length24-49
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGGCGTGATGACGAGGCAC120653914.046436144916349No Hit
AGGCTGAGGCGTGATGACGAGGCA2821863.2851881538759753No Hit
CTCATCTCCGGGGGTAGAGCACTGTTTCGGC1983242.308873067513267No Hit
GGCTGAGGCGTGATGACGAGGCAC1384511.6118361119697031No Hit
GCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGT1094961.2747441832578645No Hit
AAGGCTGAGGCGTGATGACGAGGCAC762220.8873707819124074No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCC587020.6834042617593625No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGC457050.5320941668718556No Hit
TCTCCGGGGGTAGAGCACTGTTTCGGC413790.481731200765573No Hit
GGCACGCTGATATGTAGGTGAAGCGAC335140.39016746326536195No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGC297420.34625412342419276No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGC281310.32749898278683226No Hit
GAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGC248640.2894648149021292No Hit
TCATCTCCGGGGGTAGAGCACTGTTTCGGC239130.27839334454450676No Hit
CATCTCCGGGGGTAGAGCACTGTTTCGGC216630.2521990140454No Hit
CCGGGGGTAGAGCACTGTTTCGGC196550.22882202931553047No Hit
AAGGCTGAGGCGTGATGACGAGGCA179270.20870478349221652No Hit
GAGCGGTAGTTCAGTCGGTTAGAATACCTGCC170750.19878586367655474No Hit
CGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCG168460.19611986292797898No Hit
CGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGC164670.19170757347946277No Hit
GCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTA156620.18233582412311566No Hit
CGGTGCTGAAGCAACAAATGCCCTGCTTCC142680.16610698113833575No Hit
GAGCGGTAGTTCAGTCGGTTAGAATACCTGC139960.1629403776291104No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGT126290.14702586661031974No Hit
GCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCG124790.1452795779103793No Hit
AGGCACGCTGATATGTAGGTGAAGCGAC123000.14319567339511702No Hit
GGGCTACAGCTCAGCTGGGAGAGCGCCTGC112120.1305292593582156No Hit
AAGGCTGAGGCGTGATGACGAGGC112120.1305292593582156No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGC105340.12263603443448477No Hit
GGCTTGTAGCTCAGGTGGTTAGAGCGCACCCC103700.12072675878921654No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGC102520.11935301167859673No Hit
AGGCTGAGGCGTGATGACGAGGCACT101510.1181771772873035No Hit
GGCTTGTAGCTCAGGTGGTTAGAGCGCACCC99000.11525505419606981No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGT96810.11270547269415675No Hit
TGCGAAGGTCGTAGGTTCGACTCCTATTATCGGC95500.11118038056287542No Hit
AACCGGGAGATAGCTGGTTCTCCCCGAAAG93810.10921289529427584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)