FastQCFastQC Report
Mon 25 Sept 2023
SRR1200751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1200751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8769881
Total Bases256.9 Mbp
Sequences flagged as poor quality0
Sequence length24-48
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGAGGCGTGATGACGAGGCAC115973113.224022081941591No Hit
AGGCTGAGGCGTGATGACGAGGCA4476635.104550449430271No Hit
GGCTGAGGCGTGATGACGAGGCAC2503212.854326073523689No Hit
CTCATCTCCGGGGGTAGAGCACTGTTTCGGC1073041.2235513800016216No Hit
AAGGCTGAGGCGTGATGACGAGGCAC1009381.1509620255964703No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCC929331.0596837060844955No Hit
GCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGT657290.7494856543663477No Hit
TCTCCGGGGGTAGAGCACTGTTTCGGC432670.49335903189564373No Hit
AAGGCTGAGGCGTGATGACGAGGC431540.49207053094563086No Hit
AAGGCTGAGGCGTGATGACGAGGCA396060.4516138816478809No Hit
GCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGC363030.4139508848523714No Hit
ACTCATCTCCGGGGGTAGAGCACTGTTTCGGC273940.3123645577402932No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGC265220.30242143536497246No Hit
GAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGC222400.25359523122377603No Hit
CGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCG221330.2523751462534098No Hit
GAGCGGTAGTTCAGTCGGTTAGAATACCTGCC194440.22171338470841281No Hit
CCGGGGGTAGAGCACTGTTTCGGC161730.1844152731376857No Hit
GGGCTACAGCTCAGCTGGGAGAGCGCCTGC161360.183993374596531No Hit
CATCTCCGGGGGTAGAGCACTGTTTCGGC160920.18349165741245518No Hit
GGCTTGTAGCTCAGGTGGTTAGAGCGCACCCC151180.17238546338314054No Hit
TCATCTCCGGGGGTAGAGCACTGTTTCGGC144160.1643807937644764No Hit
GAGCGGTAGTTCAGTCGGTTAGAATACCTGC143460.1635826073352649No Hit
GGCTTGTAGCTCAGGTGGTTAGAGCGCACCC142940.16298966884499344No Hit
GCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTA135170.1541297994807455No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGT119990.13682055663012987No Hit
GCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCC116870.13326292568850137No Hit
AACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGC111090.12667218631586905No Hit
CGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGC96600.11014972723119049No Hit
GGCACGCTGATATGTAGGTGAAGCGAC90360.10303446534793345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)