FastQCFastQC Report
Sat 2 Sept 2023
SRR12021931.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12021931.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141489481
Total Bases10.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA118393658.36766444849706Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG55312763.909319591044369No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA47607923.3647674486840473No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG43094883.045800980781038No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA43030023.0412168944205824No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG39771492.8109149683007177No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA35764392.5277066356614877No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG32724942.312888546110364No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT29630732.0942002041833767No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA25278381.786590764298584No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA25187681.7801803937636889No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG22919571.6198780176457075No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC22345291.579289841341633No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA22336821.5786912102674262No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA19253701.3607866721908466No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT15835971.11923302623465No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT12600260.890543940860169No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG11962470.8454670916490251No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG10576720.7475269486641201No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG10392030.7344736814745967No Hit
AAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAGATC10045670.7099941231673611No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA9648280.6819079363221355No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA8668810.6126822954421608No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG8319120.5879673839499066No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATC7588970.5363628409945188No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA6521830.4609409797750265No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG5334080.3769948099533986No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA5316300.3757381794339892No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT5104400.3607618010839972No Hit
ACGATTAAAGTCCTACGTGATCTGAGCTGTAGGCACCATCAATAGATCGG4324790.3056615919030758No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT4166210.29445369157866935No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG3898460.27553002332378335No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA3313720.2342025694475478No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGAT3290730.23257771367470068No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG3090510.21842683838807775No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGCTGTAGGCACCATCAATAGATC2939350.20774335867413352No Hit
TTTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAA2755690.194762888415712No Hit
TCTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT2745030.19400947551712341No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC2724420.19255283012876412No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCTGTAGGCACCATCAATAGAT2724010.19252385270958763No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2609360.18442077683499314No Hit
TTGCATTTATCAGTTGGTGACTCTAGATAACCTCCTGTAGGCACCATCAA2572390.18180786174486005No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT2514030.17768317349330018No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA2451550.17326729751733275No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA2311840.1633930652413659No Hit
TTGCATTTATCAGTTGGTGACTCTAGATAACCTCTGTAGGCACCATCAAT2263150.1599518200225782No Hit
TTGGCCATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA2220920.15696714584740049No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCG2202740.15568224467513594No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAG2166830.15314424681506889No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA2060550.14563273435146745No Hit
ACGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2053700.14514859942132377No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2051860.14501855441819028Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT1994240.1409461668744124No Hit
ACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCG1989990.14064579118782689No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA1987990.14050443792355136No Hit
TCATTTATCAGTTGGTGACTCTAGATAACCTCTGTAGGCACCATCAATAG1973380.13947185232801865No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1902940.1344933903602346No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT1899610.13425803717521587No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA1789190.12645392345456408No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1787280.12631893108718098No Hit
AAATTTTTTGAGGCCTTGTTCCGACAAGGCTACTGTAGGCACCATCAATA1771370.12519446586986915No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA1752630.12386998578360747No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA1748850.12360282811412673No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1700140.1201601693626963No Hit
ACGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1663500.11757057756116866No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA1656100.11704757048334921No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATC1563500.11050291434739237No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC1556530.11001029822139216No Hit
CCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATC1531340.10822995385784191No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCTGTAGGCACCATCAATAGATC1506170.10645102302693442No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG1499110.1059520460040418No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTGTAGGCACCATCAATAGATC1483010.10481415222662384No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTCTGTAGGCACCATCAATAG1466910.10367625844920583No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1445970.10219628977224109No Hit
AGATCGGAAGAGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1444790.10211289134631854No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)