FastQCFastQC Report
Fri 8 Sept 2023
SRR12021932.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12021932.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236458825
Total Bases17.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA134068525.669846325253456No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATA76632043.2408196226129435No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG75640543.1988884322672244No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA74100063.1337405148655373No Hit
AAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAGATC73194883.0954598543742233No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG64822082.741368608255581No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG57741092.44190886087673No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG53819862.276077452385209No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG48952032.070213704225249No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA48322342.0435836979228834No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA45680411.9318547320024957Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA42984171.8178289602851574No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA39615361.675359758723321No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT35625561.506628479609505No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA30931701.308122037737437No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA29418461.2441261179404068No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG24696851.0444461102265903No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT24340361.0293699124995652No Hit
TTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAG21327480.9019532258946141No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA17448800.7379212850271077No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT15362040.6496708253540547No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA14316220.6054424063047763No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGCTGTAGGCACCATCAATAGATC11917270.5039892251853996No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA11858200.5014911158422614No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG11713610.4953763091734893No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG11445260.4840276103038235No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATC11434780.4835844041769217No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG10811430.45722252066506713No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC9843910.41630546036926297No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG9682070.4094611397988635No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA9052340.3828294418700592No Hit
ACGATTAAAGTCCTACGTGATCTGAGCTGTAGGCACCATCAATAGATCGG8319010.3518164314653936No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT7765340.32840136120950447No Hit
TTGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT6915540.292462757522372No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA6647040.28110771505356164No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCCTGTAGGCACCATC6465170.27341631254405496No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT6260730.2647704098165928No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATC6035310.25523724902210776No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA6022710.2547043866939625No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG5841890.24705738937846788No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAG5697510.2409514637484983No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCTGTAGGCACCATCAATAGAT5692210.24072732324538956No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT5627950.23800972537184859No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC5347520.22615015531773872No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT5224570.22095051855222572No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCTGTAGGCACCATCAATAGATC4844510.20487752994628136No Hit
ACGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA4748620.20082227846645184No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG4574750.19346920124465644No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATA4533500.19172471147989506No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA4504150.19048348058060424No Hit
ACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATCG4333050.1832475484896789No Hit
AAAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAGAT4307980.18218732161931364No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCTGTAGGCACCATCAATAGATC4078040.17246300703727171No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGA4057460.1715926652346344No Hit
AAACCTGCATTAAAAATTTCGGTTGGGCTGTAGGCACCATCAATAGATCG4029680.17041783067305694No Hit
ATGATGACGTGCTTCATCATAACCCTTTCCTCTGTAGGCACCATCAATAG4021290.1700630120275697No Hit
TCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCG3873220.16380103385864325No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3609680.15265575306821388No Hit
ACCTGCATTAAAAATTTCGGTTGGGCTGTAGGCACCATCAATAGATCGGA3548440.15006587299078392No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA3469200.14671476101600353No Hit
AGATCGGAAGAGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3411710.14428347091718824No Hit
TTCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAG3382980.14306846022769504No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT3284630.1389091737218943No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA3250590.1374695996226827No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGAT3175680.13430160620987605No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGCTGTAGGCACCATCAATAGA3129310.13234058826097947No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA3043130.12869597909910954No Hit
AAATTTTTTGAGGCCTTGTTCCGACAAGGCTACTGTAGGCACCATCAATA2933230.12404823545917561No Hit
TCGGCGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCAT2894470.12240904944021437No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA2861370.12100922856230889No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGAT2811410.11889638714055184No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGAT2805690.11865448455983826No Hit
TAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA2754290.1164807445862932No Hit
AAATTTTTTGAGGCCTTGCTTTGGCAAGGCTACTGTAGGCACCATCAATA2736740.11573854348637654No Hit
TAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAATAGATC2730070.11545646477774724No Hit
TCGCCGAGGGCGCACCACCGGCCCGTCTCCTGTAGGCACCATCAATAGAT2711120.11465505675248111No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGCTGTAGGCACCATCAATAG2674730.11311609960000435No Hit
ATCAGTTGGTGACTCTAGATAACCTCTGTAGGCACCATCAATAGATCGGA2655500.11230285018966833No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGACTGTAGGCACCATCAATAG2612810.11049746187311892No Hit
ACGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2595240.10975441495998299No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA2468090.10437715741842157No Hit
TCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGCTGTAGGCACCAT2417330.1022304834678934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)