FastQCFastQC Report
Thu 28 Sept 2023
SRR1204655.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1204655.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499879
Total Bases24.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGGCTGGCTGGAGAAGAACAAGGACCTGGAATTCTCGGGTGCCAAGGA7860.15723805160848925RNA PCR Primer, Index 1 (100% over 22bp)
TGGCTGGAGAAGAACAAGGACCCCCTGATGGAATTCTCGGGTGCCAAGGA6190.12382996685197818RNA PCR Primer, Index 1 (100% over 22bp)
AATGTCAAGCACTGGCCCTGGATGAAACTTGGAATTCTCGGGTGCCAAGG5390.10782609391472736Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGAGCCAGATGGCACAGAAGTTGCTGTGGAATTCTCGGGTGCCAAGGA5350.10702590026786482RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)