FastQCFastQC Report
Sat 2 Sept 2023
SRR12228381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12228381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56944081
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGT48325608.486500993843418No Hit
AATACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACGTCTGA42749267.507235036421081Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACG27792314.880631930823504No Hit
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACAC23980154.211175170251672No Hit
CGCGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACG18099883.178535799006046No Hit
ACGGTTGATGTAGTGGTAACATATGAGCAGATCGGAAGAGCACACGTCTG17266743.0322273530061885Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGT16246382.8530410386287555No Hit
TTGGCGTCCGAGTTGTAATTTGAAGAAGGTAGATCGGAAGAGCACACGTC14696612.5808845698993723No Hit
GCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGTC13699232.405733793473636No Hit
CGCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGT11420062.005486751116415No Hit
AGCTCAATACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACG10379271.82271270652344No Hit
TTTGGCGTCCGAGTTGTAATTTGAAGAAGGTAGATCGGAAGAGCACACGT7557601.3271967634353428No Hit
ACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACGTCTGAACT6124491.0755270596078284Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACG5351790.9398325350092137No Hit
TTGGCGTCCGAGTTGTAATTTGAAGAAGGAGATCGGAAGAGCACACGTCT5088410.8935801422451616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCGGAGGGCATGCCTGTTTGAGCGTCGTTTAGATCGGAAGAGCACAC4732960.8311592560427834No Hit
CGCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGTC4344990.7630275041228605No Hit
CGCGCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACAC3430780.6024822843308333No Hit
ACCAAGGACGTTTTCATTAATCAGATCGGAAGAGCACACGTCTGAACTCC3422370.6010053968559085Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGCGTCCGAGTTGTAATTTGAAGAAGGAGATCGGAAGAGCACACGTC3359530.5899700093500498No Hit
ATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGTCTGAA3333730.5854392487254294Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCTTGTAGTTTAACGGTCAGAATACGCAGATCGGAAGAGCACACGTCT2741160.48137751138700435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCGTCCGAGTTGTAATTTGAAGAAGGTAGATCGGAAGAGCACACGTCT2383980.4186528183675491Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCTCGTAGTTGAACCTTGGGCTTGGCTGGAGATCGGAAGAGCACACGT2312170.4060422012956887No Hit
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAAC2268640.3983978598232185Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGTCT2224910.39071839617536364Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCTG2190960.38475640690381846Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCAAGGGCTCCGGGTTCGAGTCCCGGCTGGCGAGATCGGAAGAGCACAC2141230.37602327799442403No Hit
AAGAATGGTTTAGTGGTAAAATCCAACGTAGATCGGAAGAGCACACGTCT1937990.34033212336853763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGGAGGGCATGCCTGTTTGAGCGTCGTTTAGATCGGAAGAGCACACG1870670.32850999913406276No Hit
AAACCGGGGTTCGATTCCCCGCATTGGAAGATCGGAAGAGCACACGTCTG1801200.31631031151420286Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGTCTG1762470.3095089022509644Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1718670.301817145841725TruSeq Adapter, Index 4 (100% over 49bp)
CCAGCCGAGCCTTTCCTTCTGGGTAGCCAGATCGGAAGAGCACACGTCTG1658600.29126820046494384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGTCTGAAC1641390.2882459372730943Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGAGAAGCTGGTCAAACTTGGTCATTTAGATCGGAAGAGCACACGTCT1396860.24530380953904585Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCGGGGTTCGATTCCCCGCATTGGAGATCGGAAGAGCACACGTCTGA1169870.2054418965862317Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGTC1164940.20457613496299992No Hit
AACCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCT1133440.19904439233991678Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGTGTTCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACG1105180.19408162895806502No Hit
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAGATCGGAAGAGCACACGTC973310.17092382261819275No Hit
GCTCAATACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACGT946290.16617881672372586No Hit
TCTCTTGTAGTTTAACGGTCAGAATACGCGAGATCGGAAGAGCACACGTC940310.16512866367972467No Hit
AGCTCATTACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACG919130.1614092253064897No Hit
GCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGTCT906040.15911047892756403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTTGTAGTTTAACGGTCAGAATACGCGCAGATCGGAAGAGCACACGT890780.15643065694571487No Hit
GTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGTCTGA853350.14985754182247668Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGAGCCTTTCCTTCTGGGTAGCCAGATCGGAAGAGCACACGTCTGAA837680.14710571938108896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGGAGGGCATGCCTGTTTGAGCGTCGTTTAGATCGGAAGAGCACACGT829760.14571488123585663No Hit
ACGTCAGTGGTTTAAGGGTAAAATTCTTGCAGATCGGAAGAGCACACGTC808990.1420674433221602No Hit
AGCCCGTCGTTGCCTAGATTTGTAGATCGGAAGAGCACACGTCTGAACTC800370.1405536775630816Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATACACGGGTTCGATTCCCGTACCGAGATCGGAAGAGCACACGTCTGAA771560.13549432819892204Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACGTCTG769890.13520105803446017Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGCGTGATCGGGGTTCGATTCCCCGCAAGAGATCGGAAGAGCACACGT758690.13323421621291948No Hit
CGATTAGACCCAAGTTCGATTCTTGGTGATGAGATCGGAAGAGCACACGT737260.12947087512045372No Hit
AAGCCCGTCGTTGCCTAGATTTGTAGATCGGAAGAGCACACGTCTGAACT725860.12746891112352837Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTCAATACACGGGTTCGATTCCCGTACCGAGATCGGAAGAGCACACGT710940.1248487968398331No Hit
GATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGTCTGA696460.12230595134198408Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTATTCAATGAAGCGGAGCTGGAGGAGATCGGAAGAGCACACGTCTGA690140.12119609059982898Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGCTCCGGGTTCGAGTCCCGGCTGGCGTAGATCGGAAGAGCACACGT679460.1193205664342884No Hit
ACCAAGGACGTTTTCATTAATCAAGAACGAAGATCGGAAGAGCACACGTC675010.11853909803197983No Hit
ATTCAATGAAGCGGAGCTGGAGGAGATCGGAAGAGCACACGTCTGAACTC669360.11754689657736332Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACGTCTGAACTCC654450.1149285384024373Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTAGTGGTAAAATCCAACGTAGATCGGAAGAGCACACGTCTGAACTCC647510.11370979891658978Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGGGGTTCGATTCCCCGCATTGGAGCAGATCGGAAGAGCACACGTC643430.1129933065387428No Hit
ATGTAGTGGTAACATATGAGCAGATCGGAAGAGCACACGTCTGAACTCCA641690.11268774361289631Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGTAAGCCCGTCGTTGCCTAGATTTGTAGATCGGAAGAGCACACGTCT641060.11257710876043465Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAGAAGCTGGTCAAACTTGGTCAGATCGGAAGAGCACACGTCTGAAC633480.11124597831335623Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTCGTAGTTGAACCTTGGGCTTGGCTGGAGATCGGAAGAGCACACGTC602440.10579501669365776No Hit
AATGGCTTAGTGAGGCCTCCGGATTGGTTAGATCGGAAGAGCACACGTCT588520.10335051328688577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTTGATGTAGTGGTAACATATGAGCAGATCGGAAGAGCACACGTCTGAA587280.10313275579950092Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGCGTCCGAGTTGTAATTTGAAGAAGGAGATCGGAAGAGCACACGTCTG571990.10044766549134404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)