FastQCFastQC Report
Sat 2 Sept 2023
SRR12228382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12228382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66648535
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCTG38640195.797605303702475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGT28266594.241141984591259No Hit
GCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGTC19321052.898945940822255No Hit
ACCGAGAAGCTGGTCAAACTTGGTCAGATCGGAAGAGCACACGTCTGAAC14370312.1561329142493526Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG11904851.7862133053637264Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGT7612261.1421496361472911No Hit
CCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCTGA7180771.077408528184453Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACG6992561.049169347833377No Hit
CGCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGT6264560.9399396400836116No Hit
CGCGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACG4662890.6996237801776138No Hit
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAAC4536080.6805971053977405Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGTC4125350.6189708445954589No Hit
GCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGTCT4067450.6102834818499762Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGTTGATGTAGTGGTAACATATGAGCAGATCGGAAGAGCACACGTCTG3914800.5873797526082156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGCGTCCGAGTTGTAATTTGAAGAAGGTAGATCGGAAGAGCACACGTCT3091960.4639201746895112Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGCGTCCGAGTTGTAATTTGAAGAAGGTAGATCGGAAGAGCACACGTC2999800.4500924138842662No Hit
CTCTTGTAGTTTAACGGTCAGAATACGCGAGATCGGAAGAGCACACGTCT2780590.41720196850538427Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTCT2611160.39178055451631455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTGGATAACCGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCTG2551100.382769103626959Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAACTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTC2466600.37009065540600405No Hit
CTCTTGTAGTTTAACGGTCAGAATACGCAGATCGGAAGAGCACACGTCTG2393960.35919169116020333Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGAGAAGCTGGTCAAACTTGGTCATAGATCGGAAGAGCACACGTCTGA2278730.3419024889294266Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGCTGGTCAAACTTGGTCAGATCGGAAGAGCACACGTCTGAACT2043240.3065693792069098Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGCGTCCGAGTTGTAATTTGAAGAAGGAGATCGGAAGAGCACACGTCT1835330.27537439495106686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA1808170.27129928662347946Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCT1594650.23926257343841092Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCTATAACTTTCATTATGGGTACGTAGATCGGAAGAGCACACGTCTGA1525360.2288662458972279Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGTCCGAGTTGTAATTTGAAGAAGGTAGATCGGAAGAGCACACGTCTGA1441380.21626581889609428Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGCGTCCGAGTTGTAATTTGAAGAAGGAGATCGGAAGAGCACACGTCTG1421090.2132214909149916Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC1378510.20683275333808912Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGTC1378420.20681924966542775No Hit
GGCGTCCGAGTTGTAATTTGAAGAAGGTAGATCGGAAGAGCACACGTCTG1362100.20437058368949892Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGTGCTACTTATACTTCACCGTGATTGCAGATCGGAAGAGCACACGTC1224520.1837279694144815No Hit
TCCGATGTAGTGTAGTGGCTATCACGGTAGATCGGAAGAGCACACGTCTG1210210.18158088546132334Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTCTGAAC1206460.1810182324337662Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGAAC1170420.17561076173692342Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAGATCGGAAGAGCACACGTC1167560.17518164502790648No Hit
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACAC1167160.17512162870496703No Hit
TACTTGGATAACCGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCT1154980.17329413167146135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGTCT1130440.16961213025912722Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCAATACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACGT1127890.16922952620038836No Hit
ACTTGGATAACCGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT1123500.1685708470561281Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTACCCAATCCCGACACGGGGAGGTAGTGAGATCGGAAGAGCACACGTC1111780.16681236879400274No Hit
ACCGAGAAGCTGGTCAAACTTGGTAGATCGGAAGAGCACACGTCTGAACT1099490.16496836727168873Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGTGATCGGGGTTCGATTCCCCGCAGATCGGAAGAGCACACGTCTGAA1091960.16383855999235392Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCTGAACTCC1067570.1601790647011221Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCTCTGACGATACTGCCAGCCTAGACCGAAGATCGGAAGAGCACACGT1065770.15990899124789465No Hit
TACCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCT1050580.15762987138426976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAGAAGCTGGTCAAACTTGGTCATTTAGATCGGAAGAGCACACGTCT1031710.15479860134960205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTGATCGGGGTTCGATTCCCCGCAGATCGGAAGAGCACACGTCTGAAC1027380.15414892465378272Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTCAATACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACG1003240.15052693956438803No Hit
TTTGGCGTCCGAGTTGTAATTTGAAGAAGGTAGATCGGAAGAGCACACGT967670.14518998804699906No Hit
GTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGTCTG936420.140501212817356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCCGACTGTTTGGAAGGGATGTAGATCGGAAGAGCACACGTCTGAACT932630.13993255815750488Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTTGTAGTTTAACGGTCAGAATACGCGCAGATCGGAAGAGCACACGTC916870.13756791503369128No Hit
CTTGGATAACCGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCTGA887360.13314021080883473Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCTGACGATACTGCCAGCCTAGACCGAAGATCGGAAGAGCACACGTC861190.12921364288052242No Hit
AGGTGTCCTAAGGGGGACTCATGGAGAACAAGATCGGAAGAGCACACGTC858430.12879953025224036No Hit
TCCGATGTAGTGTAGTGGCTATCACGGTTAGATCGGAAGAGCACACGTCT836920.12557215248617243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTCTGACGATACTGCCAGCCTAGACCGAGATCGGAAGAGCACACGTC825850.12391120074882367No Hit
TCAACTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGT806250.12097040092479151No Hit
ATCAACTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACG803670.12058329564183219No Hit
AAGCTCGTAGTTGAACCTTGGGCTTGGCTGGAGATCGGAAGAGCACACGT799170.11990811200876358No Hit
TTTGGCGTCCGAGTTGTAATTTGAAGAAGGAGATCGGAAGAGCACACGTC779290.11692530075867384No Hit
ACCAAGGACGTTTTCATTAATCAAGAAGATCGGAAGAGCACACGTCTGAA764160.11465518334348986Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCACTGAATCCAACTTGGTTGATGAGTGAGATCGGAAGAGCACACGTC758270.11377144298820671No Hit
TCTCTTGTAGTTTAACGGTCAGAATACGCGAGATCGGAAGAGCACACGTC744700.1117353892324865No Hit
ACCTATAACTTTCATTATGGGTACGTTAGATCGGAAGAGCACACGTCTGA700260.10506757575391566Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGCCGCGTCCGGTGCGCTTACGACGGAGATCGGAAGAGCACACGTCTG700040.105034566776299Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGCGTCCGAGTTGTAATTTGAAGAAGATCGGAAGAGCACACGTCTGAA673110.10099396783440175Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTGGATAACCGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTGA668650.10032478583362711Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCAATGAAGCGGAGCTGGAGGAGATCGGAAGAGCACACGTCTGAACTC667940.1002182568604096Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTCTG667260.10011622911141257Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACGTCTGA667130.10009672380645726Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)