FastQCFastQC Report
Sat 2 Sept 2023
SRR12228383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12228383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79018028
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAAC1712246021.669055066775396Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG41534765.256365041152381TruSeq Adapter, Index 10 (100% over 49bp)
ACCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCTG37390714.731921429373054Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGAAACTATTGTGCTGCAATCTAGATCGGAAGAGCACACGTCTGAAC13229191.6741989562179407Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGAGAAGCTGGTCAAACTTGGTCATAGATCGGAAGAGCACACGTCTGA11496401.4549084925278064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGTC7892730.998851806324501No Hit
GCGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGT7445670.9422748439128347No Hit
ACCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG5869950.7428621225525902Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCTGA5568430.7047037417840901Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTCT4892930.6192169209790961Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAA4044480.5118426898732528Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGAAGGGATGTATTTATTAGATAGATCGGAAGAGCACACGTCTGAACT3891960.4925407655073346Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCAGATCGGAAGAGCACACGTCTGAACTCCAG3604300.4561364148444707Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTCCACGTTCTAGCATTAAGCCCAGATCGGAAGAGCACACGTCTGAACT3258830.4124160122042023Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTGAACTCCA3153290.3990595665080379Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGTCT2915190.3689272022835093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAGAAGCTGGTCAAACTTGGTCATTTAGATCGGAAGAGCACACGTCT2561360.3241488132303175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA2407170.3046355446886121Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACGCTACGTTCGGCATGGGCGAGCCACAGATCGGAAGAGCACACGTCT2374950.3005579941833021Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTTGTAGTTTAACGGTCAGAATACGCAGATCGGAAGAGCACACGTCTG2208910.2795450678672973Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTTGATGTAGTGGTAACATATGAGCAGATCGGAAGAGCACACGTCTG2174950.27524731444829276Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGAGAAGCTGGTCAAACTTGGTCAAGATCGGAAGAGCACACGTCTGAA2003300.25352442356572097Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTC1988300.2516261225855953No Hit
TGGAAGGGATGTATTTATTAGATAGATCGGAAGAGCACACGTCTGAACTC1975490.25000497354856793Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATACGCTACGTTCGGCATGGGCGAGCAGATCGGAAGAGCACACGTCTGAA1935730.24497321041724807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTGGATAACCGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCTG1923840.24346849050700176Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCATAGATTAAATTGTGTTTTGCAGATCGGAAGAGCACACGTCTGAAC1845380.2335391108469576Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCAAGGACGTTTTCATTAATCAAGAGATCGGAAGAGCACACGTCTGAAC1826550.2311561103499065Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGAGAAGCTGGTCAAACTTGGTCAGATCGGAAGAGCACACGTCTGAAC1803390.22822513363659241Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTCTGAAC1750510.22153298991465592Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGAGAAGCTGGTCAAACTTGGTCATAGATCGGAAGAGCACACGTCTGAA1709560.21635062823891277Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGTC1598900.20234622914153208No Hit
TGGGTTTGGTGTTGAGCAATACGACTAGATCGGAAGAGCACACGTCTGAA1561110.1975637762056021Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACTCGTCTGAAC1510900.19120953005812802Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGCGTCAAGCTATTGGCGAGAGTAAAATAGATCGGAAGAGCACACGTCTG1507220.19074381355100384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAACT1467720.18574495430333948Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGAAGGGATGTATTTATTAGATAAGATCGGAAGAGCACACGTCTGAACT1442720.18258111933646332Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT1429580.1809182076778732Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGGGTGCACCATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAAC1397620.1768735610562187Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCAAGGACGTTTTCATTAATCAAGAAGATCGGAAGAGCACACGTCTGAA1298240.1642966842958926Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGT1253070.15858026727774072No Hit
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGT1230440.1557163638657244No Hit
AATGGCTTAGTGAGGCCTCCGGATTGGTTAGATCGGAAGAGCACACGTCT1217250.15404712453720054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTCTG1159320.14671588615195508Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCATACGTCTGAAC1113650.1409361924344657Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGATCACACGTCTGAAC1081220.13683206571543396Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GCGTCAAGCTATTGGCGAGAGTAAAATAGATCGGAAGAGCACACGTCTGA938540.1187754267924783Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGAAGGGATGTATTTATTAGATAGATCGGAAGAGCACACGTCTGAAC936660.11853750640296921Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAACTC923860.11691762289992862Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGTACACGTCTGAAC874150.11062665345179204Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AAAGACAATTGCAAGAATACAGAGAAGATCGGAAGAGCACACGTCTGAAC870050.11010778451722433Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC859430.10876378742329536Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGCGAAACTATTGTGCTGCAATCAGATCGGAAGAGCACACGTCTGAACT838650.10613400779882788Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTTTCTCGTATG838260.10608465197334463TruSeq Adapter, Index 10 (97% over 49bp)
GCTCAATACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACGT837490.10598720585636481No Hit
AACCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCT818700.1036092674952607Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCCATAGATTAAATTGTGTTTTGCAGATCGGAAGAGCACACGTCTGAA814070.10302332525939524Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGCGTCAAGCTATTGGCGAGAGTAAAATAGATCGGAAGAGCACACGTCT801930.10148696699948016Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGTGGCAAGCCTGAACAGTCCCACAGATCGGAAGAGCACACGTCTGAA800050.1012490466099711Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)