FastQCFastQC Report
Sat 2 Sept 2023
SRR12228384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12228384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197322254
Total Bases10 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAAC4128097120.92058557166086Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC3126353115.843895134098762Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA181812749.214000768509365Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCTG54982322.7864226606695865Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTTGATGTAGTGGTAACATATGAGCAGATCGGAAGAGCACACGTCTG25294111.2818680856949871Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGGGTGCACCATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAAC21489751.0890687474105176Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGCTTAGTGAGGCCTCCGGATTGGTTAGATCGGAAGAGCACACGTCT20942751.0613475964044077Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGTC20846511.0564702955400054No Hit
GGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAACT15808730.8011630558406251Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGT13572310.6878245978276732No Hit
GTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAACTCC11233080.5692758810671198Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGTCT10353070.5246782757711657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGATCACACGTCTGAAC8102140.410604472417997Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGT8086100.40979158894059664No Hit
GCTCAATACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACGT7996460.40524876631502493No Hit
ACCGAGAAGCTGGTCAAACTTGGTCATAGATCGGAAGAGCACACGTCTGA7516630.3809316915668316Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCTGA7448500.3774789639287214Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGCCGAGCCTTTCCTTCTGGGTAGCCAGATCGGAAGAGCACACGTCTG7318920.37091204117301435Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG7103990.3600197066469756Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGTC6298560.31920170544980697No Hit
AGGGTGCACCATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAACT5642670.2859621702882028Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAA5537490.2806318034457482Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAACTC5427080.2750363879382809Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGCGTGATCGGGGTTCGATTCCCCGCAGATCGGAAGAGCACACGTCTG5171100.26206370012375796Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGAAACCACAGCCAAGGGAACGGGCAGATCGGAAGAGCACACGTCTGAA5157910.2613952504313071Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGCTTAGTGAGGCCTCCGGATTGGTAGATCGGAAGAGCACACGTCTG4865650.24658394587363675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGGGTGCACCATCGACCGATCCTGAAGATCGGAAGATCACACGTCTGAA4748910.24066773532801833Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAAGATCACACGTCTGAAC4735070.23996634459689478Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTATGCCGACTAGGGATCGGTTGTTGAGATCGGAAGAGCACACGTCTGA4701850.23828280412811423Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGAAACTATTGTGCTGCAATCTAGATCGGAAGAGCACACGTCTGAAC4344460.2201708074954384Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTTGATGTAGTGGTAACATATGAGCAGATCGGAAGAGCACACGTCTGA4299500.21789230118970768Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAACTCCAG4165500.21110137937102624Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCCGAGCCTTTCCTTCTGGGTAGCCAGATCGGAAGAGCACACGTCTGAA3757130.1904057917359894Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATGGCTTAGTGAGGCCTCCGGATTGGTTAGATCGGAAGAGCACACGTC3732680.18916670189668522No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTAGATCGGAAGAGCACACGTCT3706190.18782422787446976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCATACGTCTGAAC3697330.1873752161781002Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CTCTTGTAGTTTAACGGTCAGAATACGCAGATCGGAAGAGCACACGTCTG3629300.18392755639209352Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCACGTTCTAGCATTAAGCCCTCAGATCGGAAGAGCACACGTCTGAA3629130.18391894104351758Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAGATCGGAAGAGCACACGTC3546250.17971870522014208No Hit
CGCGCGCTACACTGACGGAGCCAGCGAGATCGGAAGAGCACACGTCTGAA3355430.17004822983625556Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACGGTTGATGTAGTGGTAACATATGAGCAGATCGGAAGAGCACACGTCT3016620.15287784012440886Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGCTTAGTGAGGCCTCCGGATTGGTTAGATCGGAAGAGCACACGTCTG3011770.15263204929738944Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGAGAAGCTGGTCAAACTTGGTCAGATCGGAAGAGCACACGTCTGAAC2968320.15043006755842148Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGAGAAGCTGGTCAAACTTGGTCATTTAGATCGGAAGAGCACACGTCT2919140.1479376978939233Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGCCGAGCCTTTCCTTCTGGGTAGCCAGATCGGAAGAGCACACGTCTGA2860300.14495577371622767Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCATACGTCTGAAC2858500.1448645523783648Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCAGATCGGAAGAGCACACGTCTGAACT2723040.13799963991897235Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGA2581380.1308205206291633Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAACTCCAG2542150.12883240224896275Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACTCGTCTGAAC2535430.12849184258760799Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TAGGGTGCACCATCGACCGATCCTGAAGATCGGAATAGCACACGTCTGAA2493840.126384122897765Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGTACACGTCTGAAC2417400.12251025674985447Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTATGCCGACTAGGGATCGGTTGTTGTAGATCGGAAGAGCACACGTCTG2400340.12164568118099847Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAACT2374010.1203113157221486Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGGCTTAGTGAGGCCTCCGGATTGGTTAGATCGGAAGAGCACACGTC2347550.11897036205556419No Hit
AACTATGCCGACTAGGGATCGGTTGTTGAGATCGGAAGAGCACACGTCTG2276330.11536103778745604Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCTCACGTCTGAAC2249140.11398308880051612Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACATGTCTGAAC2148870.1089015534963431Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAATAGCACACGTCTGAAC2141790.10854274956741575Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGTGCACACGTCTGAAC2085510.10569056240356954Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACGCGCGCTACACTGACGGAGCCAGCGAGATCGGAAGAGCACACGTCTGA2085310.10568042669936255Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGTACACGTCTGAAC2053350.104060741167086Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CGCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGT1987460.10072153341609405No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)