FastQCFastQC Report
Sat 2 Sept 2023
SRR12228385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12228385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234321427
Total Bases11.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCTG67688152.888688024249699Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGT51700022.206371848358537No Hit
GCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGTC28503791.2164397581959074No Hit
AACTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTCT13313100.5681554679162995Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGT13101040.5591055059595552No Hit
CAACTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTC13088240.5585592477635432No Hit
CCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCTGA10493330.4478177746843442Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGAGAAGCTGGTCAAACTTGGTCATAGATCGGAAGAGCACACGTCTGA10460810.44642993745510096Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG9811560.4187222707550343Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTTGATGTAGTGGTAACATATGAGCAGATCGGAAGAGCACACGTCTG8869390.37851382665060335Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGT8286240.3536270714158804No Hit
ATCCCGACTGTTTGGAAGGGATGTATTTATTAGAAGATCGGAAGAGCACA8239430.3516293881224955No Hit
CTCTTGTAGTTTAACGGTCAGAATACGCAGATCGGAAGAGCACACGTCTG7636350.32589209180601314Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGTCT7606950.32463740501204785Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTGGATAACCGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCTG7406620.3160880374802429Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTTGGGCCCCGGGTTCGATTCCCGGTTCTTGCACCAAGATCGGAAGA7067730.301625424976607No Hit
ATCCCGACTGTTTGGAAGGGATGTATTTATTAGATAGATCGGAAGAGCAC6524330.278435057499031No Hit
CGCGTGATCGGGGTTCGATTCCCCGCAAGAAGATCGGAAGAGCACACGTC6214570.2652156091555383No Hit
CTCTTGTAGTTTAACGGTCAGAATACGCGAGATCGGAAGAGCACACGTCT6082610.2595840285660261Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACG6041530.2578308811681998No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG5716290.2439508018189049TruSeq Adapter, Index 7 (100% over 49bp)
GTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTCTGAAC5677080.24227745932940228Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACG5506490.23499728857489416No Hit
AGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTGAACTCCA5112090.21816570791027148Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCTGGCTAGCTCAATCGGTAGAGCATTTGACTCAGATCGGAAGAGCACA5058010.21585776703212037No Hit
TGGCGTCCGAGTTGTAATTTGAAGAAGGTAGATCGGAAGAGCACACGTCT4850520.20700283632192118Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGAGAAGCTGGTCAAACTTGGTCATTTAGATCGGAAGAGCACACGTCT4414280.18838567417908392Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAAGAATGGTTTAGTGGTAAAATCCAACGTTGCAGATCGGAAGAGCACA4029520.17196549421833285No Hit
ACTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTCTG4010810.1711670183708808Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTGGATAACCGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCT3960770.169031490235846Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAAC3879030.16554311953725Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCAACTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGT3874100.16533272477894223No Hit
CGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGTC3764390.16065069456921666No Hit
AATCCCGACTGTTTGGAAGGGATGTATTTATTAGAAGATCGGAAGAGCAC3563450.15207529442025802No Hit
CTGTATCTAATATCTGAACAAGTTAACAGATCGGAAGAGCACACGTCTGA3526400.1504941330013324Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCAATACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACGT3370510.14384130564380695No Hit
AGATCGGAAGAGCACACGTCAGATCGGAAGAGCACACGTCTGAACTCCAG3199760.13655430666184873Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACTTGGATAACCGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCT3134220.13375729399258054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTTGTAGTTTAACGGTCAGAATACGCGCAGATCGGAAGAGCACACGTC2802600.11960493907371092No Hit
ACCGAGAAGCTGGTCAAACTTGGTCAGATCGGAAGAGCACACGTCTGAAC2719230.11604700580796651Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCAAGAATGGTTTAGTGGTAAAATCCAACGTTGCCAGATCGGAAGAGCAC2700200.11523487350561415No Hit
AACCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCT2694240.11498052203309601Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTTGTAGTTTAACGGTCAGAATACGCAGATCGGAAGAGCACACGTCT2583550.11025666893023829Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTGGATAACCGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCTGA2565570.10948934687052755Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTTGTAGTTTAACGGTCAGAATACGAGATCGGAAGAGCACACGTCTGA2493970.10643371508658489Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC2429640.10368834088740847No Hit
TTGGCGTCCGAGTTGTAATTTGAAGAAGGTAGATCGGAAGAGCACACGTC2414510.10304264662915354No Hit
CGGTTGATGTAGTGGTAACATATGAGCAGATCGGAAGAGCACACGTCTGA2389820.10198896578075209Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTCAATACACGGGTTCGATTCCCGTACCGTAGATCGGAAGAGCACACG2367830.10105051127057194No Hit
AGGTACACGGAGTCAAGACCCGCAACGCGGAGATCGGAAGAGCACACGTC2356530.10056826770690501No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)