FastQCFastQC Report
Fri 22 Sept 2023
SRR12228386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12228386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625524379
Total Bases31.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAAC21236187233.949415742915434Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC10723040117.14248150830265Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCTG269911154.314958122519474Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA126269292.0186150090882387Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGAGCACACGTCTGA124622081.9922817428671313Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG91460721.4621447711792541Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCACGTTCTAGCAGTAAGCCCTAGATCGGAAGAGCACACGTCTGAAC63693271.0182380117913838Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAA57922350.9259806962695534Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG53969480.8627877955177188TruSeq Adapter, Index 12 (100% over 49bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGATCACACGTCTGAAC50770970.8116545366491623Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGCGCACACGTCTGAAC36490370.5833564801796478Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAATAGCACACGTCTGAAC35184560.5624810348119142Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCATACGTCTGAAC29861060.4773764381132138Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCTCGATCGGAAGAGCACACGTCTGAAC29196140.4667466365847269Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACTCGTCTGAAC25195060.4027830224663394Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCAGATCGGAAGAGCACACGTCTGAACT23702050.3789148879839262Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGTGCACCATCGACCGATCCTGACGATCGGAAGAGCACACGTCTGAAC23231330.3713896816801764Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAAGCGCACACGTCTGAAC22109460.35345480915300986Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAAGATCACACGTCTGAAC21386460.34189650664278903Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAATAGCACACGTCTGAAC19160190.3063060472659851Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAACT19042930.3044314600566511Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCAAGGACGTTTTCATTAATCAAGAAGATCGGAAGAGCACACGTCTGAA18790690.3003990033136662Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGTACACGTCTGAAC16800830.26858793300524586Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGGGTGCACCATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAACT15807470.2527074967928628Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCATACGTCTGAAC14319440.22891897551446191Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGACGAGCACACGTCTGAAC13800780.22062737222268997Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACCAAGGACGTTTTCATTAATCAAGAGATCGGAAGAGCACACGTCTGAAC13342240.21329688255044013Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACTTCTGAAC13331080.21311847223783426Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACTCGTCTGAAC11146950.17820168764357625Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TTGCCGCGTCCGGTGCGCTTACGACGGAGATCGGAAGAGCACACGTCTGA11013380.17606635919780833Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGTGCACACGTCTGAAC10260650.16403277545158637Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAACT10082920.16119147931722738Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCTATAACTTTCATTATGGGTACGTAGATCGGAAGAGCACACGTCTGAA9859330.1576170382961205Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAAGATCACACGTCTG9839980.15730769783474738Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TAGGGTGCACCATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAAC9829910.15714671290213617Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGTGATCGGGGTTCGATTCCCCGCAAGAGAAGATCGGAAGAGCACACGT9619120.15377690019656293No Hit
AGGGTGCACCATCGACCGATCCTGAAGATCGGACGAGCACACGTCTGAAC9607780.15359561229826982Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
NCTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAAC9327940.1491219257499155Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGTGCACCATCGACCGATCCTGAAGATCGGAAGAGTACACGTCTGAAC8796760.1406301703870122Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTTTAATCTCGTATG8271190.13222809977802638TruSeq Adapter, Index 12 (97% over 49bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACATGTCTGAAC8216960.13136114715682407Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GCGTGATCGGGGTTCGATTCCCCGCAAGAGAGATCGGAAGAGCACACGTC7878870.12595624190692015No Hit
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCTCACGTCTGAAC7827740.12513884770588612Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACTCCACGTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCAGAAC7500720.11991091397574451Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGGGTGCACCATCGACCGATCTTGAAGATCGGAAGAGCACACGTCTGAAC7428100.11874996801683407Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCCACTTTCTAGCATTAAGCCCTAGATCGGAAGAGCACACGTCTGAAC7354970.11758086889847662Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGAGAAGCTGGTCAAACTTGGTCATTAGATCGGAATAGCACACGTCTG7275760.11631457133024067Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTGCCGCGTCCGGTGCGCTTACGACGGTAGATCGGAAGAGCACACGTCTG7213880.1153253213173327Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)