FastQCFastQC Report
Sat 2 Sept 2023
SRR12228555.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12228555.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21785312
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC6735303.0916702042183286Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC4288351.9684592995500823No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG2533751.1630542633495449Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2177740.9996368195231723Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2165050.9938117939279456Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT2114170.9704566085626867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA2016620.9256787325331856No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA1830450.8402220725597136Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1728950.7936310482952917No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1613520.7406458075973389No Hit
CCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTGA1580860.725654055356196Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1502550.6897078178178031Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1399250.6422905487881009No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1321330.6065233309488521No Hit
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1209060.554988608838836Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1187390.5450415399145994No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC1172340.5381332156271161No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA1159000.5320098238666493Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA1064630.4886916469224769No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG1014200.4655430227485381TruSeq Adapter, Index 3 (100% over 49bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG996800.4575559900174943No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT996150.4572576238522542No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC995760.4570786041531101No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG954140.4379739890803491Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT940050.4315063286676821No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT846570.38859668385745405No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC831940.38188115001520295No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA802790.36850057506635664No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGTC802170.3682159796472045No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA800950.36765596930629224No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC765660.35145698165810063No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT757210.34757822150997886Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA745160.34204697183129623Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG742440.34079842418598366No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGT735330.3375347573631261No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC674250.309497518327945No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC664120.3048475963988948No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA639690.29363361883456157No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT633740.29090242086044027Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC617760.28356720344422887No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC594640.27295454845907186No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC580740.2665741027716289No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT574830.26386126579229163No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC566130.2598677494267697No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG561730.2578480400005288No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGAGATCGGAAGAGCACACGTCTGA552370.2535515672210708Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC548330.2516971067478859Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT543460.24946165563293288Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC499830.2294344005722755No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG498260.22871373152700314No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT492890.22624876797725002No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC488120.22405921934925696No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT487510.2237792141788008No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACACG479500.22010242497330312No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAGATCGGAAGAGCACACGTCT478670.21972143433153496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC461940.21204194826312334No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT458060.21026093176907448No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG453470.20815400761760952Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA452930.2079061341880254Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC452730.20781432921410536Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT450780.20691923071838492Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG448170.20572117580872837Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAA440760.2023198015249908Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC439910.2019296303858306No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC432720.19862924157340506Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTAGTGTAGTGGTTATCACATTCGCAGATCGGAAGAGCACACGTCTG432570.198560387842965Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC425380.19525999903053948No Hit
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA414960.19047695988930524Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG395910.18173253612342113No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT390390.17919871884322794No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT383040.17582488605166638Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC375810.17250613624445682No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC354020.16250398433586813No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTC348760.160089513521771No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT338320.15529729388314475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACACG329500.15124869453327086No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCTG322030.14781977875735725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC317160.1455843276424042No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG311410.14294493464220298No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT306770.14081505924725796Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA291080.13361295904323062Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG290470.13333295387277447No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTATAGATCGGAAGAGCACACG282480.12966534516466877No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACA274150.12584166800089894No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTC270000.12393671479205806Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTGA261390.1199845106648002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT260280.11947499305954397Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC253770.11648674115844657No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACAC253570.11639493618452652No Hit
TCCGTAGTGTAGTGGTCATCACGCTCGCAGATCGGAAGAGCACACGTCTG251640.1155090181861981Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC247940.11381062616867732No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA247310.11352144050082917No Hit
TCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTG245150.11252994678249272Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG243050.11156599455633227Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCAC241040.11064335456843584No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCAC240080.11020269069361964No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACACGTC233680.10726493152817825No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC232550.10674623342553Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC228960.10509833414366523Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTCT227220.10429963087056086Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC225910.10369830829138457No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG225540.1035284690896325No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCTG223900.10277566830348817Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT221970.10188975030515973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)