FastQCFastQC Report
Sat 2 Sept 2023
SRR12228559.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12228559.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19563287
Total Bases997.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT4468042.283890227649372Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC3533301.806087085467795Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG2041381.043474953876616Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1878330.9601300640326955No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT1650670.8437590267934013Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1647800.8422919931604541Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1586340.8108760046305101No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC1434220.7331181104688593No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1275190.6518280900341543Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT1264350.646287098890897Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1234350.6309522525534692Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1205940.616430153071925No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1203830.6153516022128592No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1200510.6136545458848506No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1166230.5961319281366163No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG994960.5085852903962407No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA992820.5074914046908374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC946550.48383996002307794No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC932280.4765456847819081No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC890560.4552200251419917No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC887000.45340029004328364No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC874920.447225458584746No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA829810.4241669613087003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC820920.41962273517737586No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA818850.41856463078009337No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT681060.3481316815522872No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC622290.31809071757726604No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT621070.3174671004928773Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC597930.3056388223512746No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC583750.29839055164911704No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGTC573420.29311025289359605No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT558300.2853814903395324Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTCT554340.2833572906229919Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA547160.27968715073290085Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA505650.2584688350173465Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC496400.2537405907299729No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT493050.25202819955562683No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT452590.23134660346188243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTGA451800.23094278584166353Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACACG450110.23007892283132175No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT439870.22484462861481302No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT439650.22473217307500526No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT433240.2214556275742415Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG427180.21835798861408107Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC425750.21762702760533031No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGT421050.21522456834579995No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC413210.2112170618362855Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA408150.20863058442070598No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC401370.2051649091484473No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT397860.20337073212696824No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC390680.19970059223687717No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT390370.19954213215805705Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC386770.19770195059756573No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA378930.19369444408805125Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTC369570.18890997203077378No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT360690.18437085751489513No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA353050.18046558331429682No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA351450.17964772484296734Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT338220.17288505760816164No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG335930.17171449767107133Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC335070.17127489874273172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT335070.17127489874273172Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAGC334550.17100909473954964No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGT325940.16660799384070785No Hit
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAAC320530.16384260988452504Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA319690.16341323418707707Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA312880.15993222406848093Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA311760.15935972313855026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT307860.15736619311468467No Hit
AGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGTC306720.15678346895386241No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG305240.15602694986788262No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC303230.15499951516327498No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG299940.1533177936816037Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACA295700.15115046873258056No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG292480.14950452855903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT287500.14695894406701696No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT277900.14205179323904005No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG262670.1342668029150725No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT243520.12447805933634773No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT240850.12311325801231664No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT239380.12236185054178268No Hit
TCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTC238080.12169734053382747Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAA236820.12105327698765549Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG231030.11809365164453192No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC229270.11719400732606948No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC221290.11311493820031367No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG220790.11285935742802321No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA218690.11178591818440325No Hit
CCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGTCT216600.11071759055622914Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTG213720.10924544530783604Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG212330.10853493076086856TruSeq Adapter, Index 6 (100% over 49bp)
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC205270.1049261302561272Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG204010.10428206670995523Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC203740.1041440530929184No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC203320.1039293652441944No Hit
AAGGTGAGTTGAGAAGCTTGCTGGAGGTAGATCGGAAGAGCACACGTCTG203260.10389869555151954Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)