FastQCFastQC Report
Sat 2 Sept 2023
SRR12228560.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12228560.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22284942
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC3816041.7123849817513546Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3767841.6907560270966828Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC3562841.5987656597894668Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT2251641.0103862958225334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG2166940.9723785684521862Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG2095950.940522977353946No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA2087580.9367670779668171Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1887420.8469485807950499No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1718580.7711844168138288No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1641980.7368114307858642No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1601660.7187184961037816No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC1411590.6334277199375256No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1350360.605951767790107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1283000.575725079293453No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1238940.5559538813248874No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1223140.548863892039746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA1209030.5425322623680151Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1167030.5236854554075124TruSeq Adapter, Index 7 (100% over 49bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT1104500.4956261497113163Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1030470.4624064087759349No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1020790.4580626685050381No Hit
CCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTGA1003870.4504700977009498Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT977890.4388120013953817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC972870.43655935923010253No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC967860.43431120439981397No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC948340.42555192649817086No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGTC935460.4197722390302834No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG836100.37518607856372255No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG829330.3721481527750891Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT816180.3662473072624555No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA697790.31312174830879075No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC695220.3119685032162076No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC689900.3095812410012106No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT649220.2913267622594665No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA645240.28954080293320933Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA640410.2873734201327515Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC639240.2868484019388518No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC613000.2750736349235282No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC603380.2707568186625749No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACACG594940.266969507930512No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT592110.2656995921281734No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT590330.2649008464998473No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA582280.2612885418324176Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT565480.25374981904821653Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT559520.25107536739382136Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT549760.24669572844299975No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC548940.2463277669737709No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGT540860.2427020003013694No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC532760.23906725895898673No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC517940.23241702850292362Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC507990.22795213018728072No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA507060.2275348080331553Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTCT506270.2271803085688982Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT482860.21667545735591323Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC460400.20659690296703487Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC454420.20391347664265852Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC445260.19980307779127268No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT420950.18889436642913407No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGT417270.18724302715259478No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC407490.18285441353179202No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTC400060.17952032363377926No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC397080.17818309780658168No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA391400.17563429153192323No Hit
TGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAA381360.17112900720136495Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG379330.17021807819827398No Hit
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAAC368200.16522367435374075Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACA360900.16194791981060574No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAGC358640.16093378210273107No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT353560.15865421592750836No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG351660.15780162227929515Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC350830.15742917347507568No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT349930.15702531332592204No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA348100.156204131022643Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG341020.1530270978493011No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG337020.15123216385306276Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG326220.1463858420632192No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT324220.1454883750651Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT322590.1447569394616329No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG312340.14015742109627208Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT311530.13979394696203382Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT311170.1396324029023724Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC310940.13952919419758866No Hit
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC310170.13918366940331278Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTGA308790.13856441717461054Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT297730.1336014246750115No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA297410.1334578299553124No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT278300.12488253278828368Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT278130.12480624809344354No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC274840.1233299148815375No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA272180.122136283774039Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTGA269310.12084841863173797Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTG267760.1201528817081956Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA266410.11954709148446516No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA266330.1195111928045404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC264870.11885604189591338No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC263260.11813358096242745No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA254440.11417575150072187No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG253180.11361034729190679Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG252840.11345777790222654No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG249860.11212055207502895No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC247090.1108775602826339Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG245920.11035254208873418Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG245360.1101012513292608Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGTCT241810.10850824740759926Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT240790.10805053923855848No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC240700.10801015322364312No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG236530.10613893453256464Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT225940.10138684677752359Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)