FastQCFastQC Report
Sat 2 Sept 2023
SRR12228561.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12228561.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22498055
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC3873631.7217621701075936Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3808811.6929507906350127Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC3619181.608663504467386Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT2302631.023479585235257Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG2200200.9779512051152865Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA2130190.946832959560282Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG2128600.9461262318009268No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1914410.8509224464070339No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA1743440.7749292105473118No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1670760.7426241957360314No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1638900.7284629715768762No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC1439010.639615291188505No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1389250.6174978237007599Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1316150.5850061260851216No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1249230.5552613325907506Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1245000.5533811700611453No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA1225090.5445315161688421Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1172220.5210317069631131TruSeq Adapter, Index 7 (100% over 49bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT1120930.4982341806880639Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1050420.46689369369929984No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1036300.4606175956099316No Hit
CCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTGA1021990.4542570457757349Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1004460.44646526110812695Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC988320.43929130762637036No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC984550.43761560721582377No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC973850.43285964053337056No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGTC959570.4265124251851994No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG856510.38070402085869204No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG850980.378246030601312Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT825000.36669836570316855No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC715090.3178452537341561No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA706870.31419160456315No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC699880.31108466931919226No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT659580.29317200975817687No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA652300.2899361744826386Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA651970.2897894951363573Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC648070.28805601195303326No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC624790.27770845079719114No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT612180.27210352183777664No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT609950.27111232504320926No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC602310.2677164759353642No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACACG601750.26746756552955353No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA592010.26313830239991853Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT572710.25455978305680205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGT564600.25095502700122296No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT562030.2498127060316992Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC561490.24957268528323892No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT554170.2463190706929999No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC548740.24390552872237178No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC524510.2331357088423866Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC512720.22789525583433767No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA511210.227224086704384Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTCT509740.22657069688913106Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT496910.2208679816988624Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC464740.2065689678507764Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC463200.2058844642347972Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC457910.2035331498656217No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT437070.19427012690652593No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGT431390.19174546421901806No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC421530.18736286314528078No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTC406020.18046893387006122No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC405650.1803044752090792No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA397720.1767797260696536No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG387690.1723215629084381No Hit
TGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAA385190.1712103557396406Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAAC373840.16616547519330005Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACA362840.1612761636505911No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT361610.16072944972354278No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAGC361280.1605827703772615No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC357440.15887595616598857No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT356030.15824923532278679No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA355650.15808033183312958Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG354400.15752472824873084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG347800.1545911413231055No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG343650.15274653742290167Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT332880.14795945693972212Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG330640.1469638153164796No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT327140.14540812528016311No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT322360.14328349717342231Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC319880.14218117966197522No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG317570.14115442423800637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC315900.14041213784924964Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT315190.14009655501331114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTGA313680.13942538588335748Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA307530.13669181624811566No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT304070.13515390552649995No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT288270.12813107621969988Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT284150.12629980680552164No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC282070.12537528244108212No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA278380.12373514065993706Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTGA276280.12280172663814716Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACACGTCTG275390.12240613688605526Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA273610.12161495738187145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC270680.1203126225800408No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC268740.11945032581705396No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA268520.11935253958619979No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG261270.1161300387966871Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG260780.11591224219160277No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA257890.11462768670447289No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG253850.11283197591969617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG252920.1124186068529035No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC252700.11232082062204933Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG250080.11115627550914955Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGTCT245820.10926277849351866Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT244610.10872495422382068No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC243870.10839603690185663No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG242410.10774709191527888Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT231400.1028533355438948Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTG225100.10005309347852515Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)