FastQCFastQC Report
Sat 2 Sept 2023
SRR12228562.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12228562.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18724122
Total Bases954.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC4126902.2040552822717134Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3108711.6602701050548592Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2732381.4592833778801484Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA2157251.1521234480313682No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC2087351.1147919245559283No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1673080.8935425650398988No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1569220.8380740095583653No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT1554720.8303299882365646Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1488760.7951027022789107No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC1486020.7936393492843082No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1356810.724632108250523Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1309040.6991195635234592Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1304070.6964652334566075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1269850.6781893431371575No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1195070.6382515559341047No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC1151600.6150355140817818No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1136420.6069283248635103No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA1027760.5488962312892428No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC951490.5081626791365704No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC941710.5029394702726249No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG898750.4799958043426549No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC890750.4757232408547648No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT826380.441345126890329Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGT808950.43203627919108833No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA802370.42852209572229877Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC656580.35065996685986134No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC638480.3409932919685099No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT615130.32852274728823067No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA609870.3257135367949429Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC607140.3242555245047004No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA593730.3170936399581246Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTGA576900.3081052345204758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTC561740.30000872671092405No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACACG551090.2943208765676703No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA548870.29313524019978077Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC534650.2855407586000561No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT524950.28036027537098934No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT521490.2785123916624769No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT515380.2752492212986008No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA514130.274581633253618Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC482390.2576302376154139Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT481840.25733649887562154No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAGC481140.25696264957043113No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACA456330.24371236205361194No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG440160.23507644310371403Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTCT435070.23235802458454394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAG428460.22882781900267474No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG410920.21946022355547568Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG409370.21863241437969694Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT394610.21074953474453967No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC392710.20973480091616578Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC390970.20880551835754968No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC380690.2033152742756109No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA380060.20297880990093956No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC375090.20032447983408783No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT371660.1984926182386549No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC371230.19826296795118084Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT369110.19713073862688996No Hit
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC357810.19109574270024515Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC355540.18988340281055635No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA352390.18820108093719962Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG340530.18186700556640253No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA335040.17893495887283795Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA333720.17822998589733607Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG330280.17639278359754332No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT322200.17207749447477433Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT321470.17168762305650434No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT316290.16892113819809548No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA307730.16434949526605305No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG307250.16409314145677964No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT302370.16148687772916667Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACG296960.158597556670481No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC290730.15527029785428656No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG282650.15095500873151757No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA280130.14960915123283217Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT277830.14838078923006376No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT271310.14489864998743332Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT269100.14371835432390367No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG268470.14338188994923234No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG262690.1402949628292317No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT262660.14027894071615213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC262630.14026291860307255No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT261240.13952056069705165Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACA259700.1386980922256328No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC256410.136941000491238No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT255760.13659385470784693No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC249920.1334748833616871No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG248640.13279127320362472Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACG246050.1314080307744203No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT239770.12805406843642655No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC239250.1277763518097137No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT231120.12343435916514536No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGAGATCGGAAGAGCACACGTC225630.12050231247158077No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA225400.12037947627130394No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA225350.12035277274950462No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG224970.12014982598382985No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC223970.11961575554784358No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC223550.11939144596472935No Hit
TGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAA222280.11871317651102681Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC218510.11669973096735857No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC214100.11434448034465916No Hit
TCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTC212280.11337247215116415Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACAC209070.11165810605164823No Hit
AGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGTC208300.11124687181593883No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACG200430.10704373748472691No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGT197960.10572458350784084No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC197630.1055483402639654No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGCA197350.10539880054188921No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC194730.10399953599960522No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA191930.10250413877884368Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCT191740.10240266539600629Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAAC190610.1017991658033418Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGC189060.1009713566275631No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)