FastQCFastQC Report
Sat 2 Sept 2023
SRR12228563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12228563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18842587
Total Bases960.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC4181702.2192812483763507Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT3151581.6725834939756414Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2776571.4735609287620643Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA2187921.1611569048347767No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC2114661.12227689329496No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1706130.9054648387718736No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA1612860.8559652663405508No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT1582340.8397679150957351Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1516270.8047037277843005No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGTC1500480.7963237744371301No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1376860.730717071917991Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1341120.7117494004406083Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1324650.7030085624654406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1277420.6779430022002817No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1198910.6362767490472513No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC1160200.6157328608858221No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1151220.610967060945506No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACA1044610.5543877812531792No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC964120.5116707169774511No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC949750.504044375647569No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG918240.48732161884140435No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC905690.4806611746041029No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT839960.44577742960666705Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGT821870.43617683707656496No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA814760.43240346986324113Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC659890.3502119958368774No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC654360.34727715467095893No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT623410.3308515969702037No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA614330.32603272576106457Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC612430.3250243716534253No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA604450.3207892844013404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTGA589860.31304618628004743Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTC581240.30847144290749456No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGA560090.297246869551405Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACACG554640.29435448540054504No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC542550.28793816899983No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT536790.28488126391561835No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT534860.2838569884273322No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACGT523340.27774317825890893No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA516850.2742988529122885Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT491270.2607232223473348No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAGC490960.26055870141398313No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC485940.2578945237190626Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACA454870.24140528049571963No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTAGATCGGAAGAGCACACGTCT441610.23436803024977407Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG440750.23391161733789528Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAG436990.231916137630146No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG414420.21993795225676815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG409790.21748075251025772Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT397710.21106974323642502No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC395670.2099870893524334No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC394830.2095412906943192Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC391490.20776871031562705No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA386660.2052053680314704No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC378270.20075268857721074No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT377410.20029627566533195No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC376870.2000096908136871Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT376590.19986109126098237No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACAC361340.19176772276545678No Hit
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC359820.19096103947934537Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA355020.1884136185758357Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG349660.18556899856691653No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG340380.1806439848201311No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA339110.1799699797060775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA338110.17943926701784632Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT330780.17554914301311175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT329560.1749016735334697No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACGT328450.17431258244953307No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA313610.16643680615618228No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG312990.16610776428947893No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTCT307660.1632790656612067Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACG300510.15948446994035373No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC292810.1553979822409736No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA287700.15268604040411224Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACACG283750.15058972528559905No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT283570.15049419700171743No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT274620.14574431844204833No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG272050.14438038683329418No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT272010.14435915832576493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT267770.1421089365276647Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG266370.14136593876414102No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC266290.14132348174908255No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT266160.1412544890996125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACA263090.13962520114674273No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC259010.1374598933787595No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT258560.13722107266905548No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG256630.1361967971807693Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACTGGCGATGCGGCGGCGTTAAGATCGGAAGAGCACACG254000.1348010228107213No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC252910.13422254598054928No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGT245760.1304279502596963No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCAC242560.1287296696573565No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT230450.12230273900287685No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC230390.12227089624158297No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG230070.12210106818134898No Hit
TGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAA229160.1216181196350586Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGAGACCGGGGTTCAATTCCCCGACGGGAGATCGGAAGAGCACACGTC229100.12158627687376473No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA228580.12131030627588452No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC228010.12100780004359274No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA227340.12065222254247784No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC220010.11676209853774325No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC218100.1157484373032217No Hit
TCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTC213020.11305241684700727Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACAC212630.11284543889859709No Hit
AGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGTC212020.11252170415877608No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGT202600.10752239063563832No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC201990.10719865589581727No Hit
AAAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACG201680.10703413496246561No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGCA199400.10582411003329852No Hit
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAAC199110.10567020335371145Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC197220.10466715637295451No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCT196600.10433811450625119Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA195410.10370656640725608Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGC190620.10116445263062869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)