FastQCFastQC Report
Thu 29 Jun 2023
SRR1223686.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1223686.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23135864
Total Bases971.7 Mbp
Sequences flagged as poor quality0
Sequence length42
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAA14173706.1262894698896915No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAA13437675.808155684179333No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAA11220604.849872907275043No Hit
AGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAA11015364.761162150676543No Hit
AAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAA3568041.5422116934988899No Hit
AAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAA3404901.4716977935209163No Hit
AGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAA2908361.257078620448322No Hit
AAGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAAAAAAA2631231.1372948941954362No Hit
CACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAA2589161.119111004456112No Hit
AGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAA2521461.0898490758763104No Hit
AGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAAAAAAAA2193560.9481210643354405No Hit
AAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAA2017420.8719881825031475No Hit
AGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAA1348960.5830601355540471No Hit
AGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAAA1291430.5581939797018171No Hit
AAGAGGTGCACAATCGACCGATCCTTAAAAAAAAAAAAAAAA1069050.4620748116430836No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAA1052360.4548609034008845No Hit
AGTTTCTTTACTTATTCAATGAAGCGAAAAAAAAAAAAAAAA969800.41917604633222255No Hit
ATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAAAAAA816610.3529628286196703No Hit
TATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAAAAA809300.3498032319000492No Hit
AGAGGTGCACAATCGACCGATCCTTAAAAAAAAAAAAAAAAA790300.3415908738052748No Hit
AAGAGGTGCACAATCGACCGATCCGAAAAAAAAAAAAAAAAA779720.33701788703460567No Hit
AGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAA709050.30647223721577893No Hit
GAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAA694270.3000838870767913No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAA685620.29634510299680183No Hit
AATATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAAA619250.2676580394836346No Hit
GTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAAA612220.26461946698856803No Hit
AGAGGTGCACAATCGACCGATCCGAAAAAAAAAAAAAAAAAA608790.2631369202377746No Hit
GAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAA601770.2601026700364421No Hit
AAGAGGTGCACAATCGACCGATCCTGGAAAAAAAAAAAAAAA579240.25036454225353333No Hit
TTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAAAA572610.24749886150783043No Hit
CCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAA562940.24331920346696367No Hit
GTTTCTTTACTTATTCAATGAAGCGAAAAAAAAAAAAAAAAA537430.23229303215129551No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAA535370.23140263964207258No Hit
AAGAGGTGCACAATCGACCGATCCTGTAAAAAAAAAAAAAAA516690.22332859494678914No Hit
AGAGGTGCACAATCGACCGATCCTGGAAAAAAAAAAAAAAAA493250.21319713843407792No Hit
CACGTTCTAGCATTCAAGGTCCCGAAAAAAAAAAAAAAAAAA493170.21316256008420523No Hit
ATATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAAAA489710.2116670464522094No Hit
TTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAA486110.21011102070793639No Hit
CACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAA426320.18426802647180152No Hit
AGAGGTGCACAATCGACCGATCCTGTAAAAAAAAAAAAAAAA423590.18308804028239448No Hit
AGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAAAAAAAA410530.17744312466567058No Hit
AAGAGGTGCACAATCGACCGATCCAGAAAAAAAAAAAAAAAA405970.1754721587229247No Hit
CTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAAAAAAA396190.17124495545098292No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAA391860.1693734022641212No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAA383280.16566487424027043No Hit
ATTAAGCCTTTGTTGTCTGATTTGTAAAAAAAAAAAAAAAAA377550.16318819993063582No Hit
AGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAAAA366850.15856334563515762No Hit
CACGTTCTAGCATTCAAGGTCCCTAAAAAAAAAAAAAAAAAA364100.15737471485828236No Hit
AGTTTCTTTACTTATTCAATGAAGCAAAAAAAAAAAAAAAAA362540.15670043703576406No Hit
AGAGGTGCACAATCGACCGATCCAGAAAAAAAAAAAAAAAAA358550.1549758418358614No Hit
TTTCTTTACTTATTCAATGAAGCGAAAAAAAAAAAAAAAAAA347820.15033802065918092No Hit
AAGAGGTGCACAATCGACCGATCCGGAAAAAAAAAAAAAAAA328610.14203489439599057No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAA328280.14189225870276553No Hit
AGGTGCACAATCGACCGATCCTCAAAAAAAAAAAAAAAAAAA320180.1383912007781512No Hit
TGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAA305040.1318472480647362No Hit
CAATATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAA303360.1311211027174088No Hit
AGAGGTGCACAATCGACCGATCCGGAAAAAAAAAAAAAAAAA286600.12387693841907092No Hit
TTTCTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAAAA282880.12226904514998878No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAA264180.1141863558672371No Hit
ATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAA264130.11416474439856665No Hit
TAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAA260260.11249201672347313No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAAA255580.11046918325591816No Hit
TGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAA247820.10711508331826293No Hit
TTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAAAAA241170.1042407579850919No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAA233850.1010768389717367No Hit
CACGTTCTAGCATTCAAGGTCACAAAAAAAAAAAAAAAAAAA232930.10067918794820026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)