FastQCFastQC Report
Wed 28 Jun 2023
SRR1223688.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1223688.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41451837
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA6075751.465737212080613No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAA3888450.9380645784166333No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAA2529110.6101321878690201No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAT2482820.5989650108872135No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAA1611080.38866311280728044No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAATC1320820.31863967814019917No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAAA1080210.26059399973033764No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGAAAAAAAAAAAAAAAAAAAAA1060020.2557232867629003No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAT923920.22289000123203226No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAA732140.17662425913717647No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAA731920.17657118549414347No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAA708990.17103946442711332No Hit
ATCTTTGAACGCACATTGCGCCCCTTGGTAAAAAAAAAAAAAAAAAAAAA675080.16285888608507268No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAAATC599230.1445605414302869No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTAAAAAAAAAAAAAAAAAAAAAATC575340.1387972262845673No Hit
TCTTTGAACGCACATTGCGCCCCTTGGTAAAAAAAAAAAAAAAAAAAAAA520350.12553122796463762No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAAAAAAAAAAAAAAAAAAA511830.1234758305162688No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCAAAAAAAAAAAAAAAAAAAAA506400.12216587650868162No Hit
ATCTTTGAACGCACATTGCGCCCCTTGGAAAAAAAAAAAAAAAAAAAAAA484590.11690434853345581No Hit
ATCAGCTTGCGTTGATTACGTCCCTGCCCTAAAAAAAAAAAAAAAAAAAA472580.11400701011151809No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGGAAAAAAAAAAAAAAAAAAAA454620.10967427088936976No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAAAT437130.10545491626824643No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCACTAAAAAAAAAAAAAAAAAAAA422410.10190380706167497No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAATCG421130.10159501495675571No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCACAAAAAAAAAAAAAAAAAAAAA419240.10113906411433587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)