FastQCFastQC Report
Thu 29 Jun 2023
SRR1223697.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1223697.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19360675
Total Bases813.1 Mbp
Sequences flagged as poor quality0
Sequence length42
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAA4422762.2844038237303192No Hit
CACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAA3208911.6574370470037847No Hit
TCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAA1647840.8511273496404438No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAA1497160.7732994846512324No Hit
TCACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAA1455220.7516370167878961No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAA1146490.5921746013504178No Hit
CACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAA1104620.5705482892512788No Hit
ATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAA1084130.5599649805598204No Hit
CACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAA1042090.5382508616047735No Hit
TCACGTTCTAGCATTCAAGGTCAAAAAAAAAAAAAAAAAAAA1035110.5346456154033886No Hit
TCACGTTCTAGCATTCAAGGTCACAAAAAAAAAAAAAAAAAA940410.48573203155365186No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAA927080.4788469410286573No Hit
AACTATGCCGACTAGGGATCGGGTGGCGAAAAAAAAAAAAAA808960.41783667150034803No Hit
CCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAA789540.4078060294901908No Hit
CACGTTCTAGCATTCAAGGTCACAAAAAAAAAAAAAAAAAAA749630.38719207878857526No Hit
TCACGTTCTAGCATTCAAGGTCCCTAAAAAAAAAAAAAAAAA742730.38362815346055856No Hit
AACTATGCCGACTAGGGATCGGGTGGTAAAAAAAAAAAAAAA720300.37204281358991875No Hit
TCACGTTCTAGCATTCAAGGTCCCGAAAAAAAAAAAAAAAAA678960.3506902522768447No Hit
TCACGTTCTAGCATTCAAGGTCCTAAAAAAAAAAAAAAAAAA653990.337792974676761No Hit
CACGTTCTAGCATTCAAGGTCCCTAAAAAAAAAAAAAAAAAA607690.31387851921485177No Hit
ACTATGCCGACTAGGGATCGGGTGGCGAAAAAAAAAAAAAAA587340.30336752205178796No Hit
CACGTTCTAGCATTCAAGGTCCCGAAAAAAAAAAAAAAAAAA540060.2789468858911169No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAA528990.27322911003877703No Hit
CACGTTCTAGCATTCAAGGTCCTAAAAAAAAAAAAAAAAAAA524990.2711630663703615No Hit
ACTATGCCGACTAGGGATCGGGTGGTAAAAAAAAAAAAAAAA494390.25535783230698306No Hit
CACGTTCTAGCATTCAAGGTCAAAAAAAAAAAAAAAAAAAAT489430.2527959381581479No Hit
ATGATTAATAGGGACGGTCGGGGACAAAAAAAAAAAAAAAAA479700.24777028693472722No Hit
AGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAA434290.2243155261890404No Hit
GCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAA422050.21799343256368903No Hit
TCACGTTCTAGCATTCAAGGTCCGAAAAAAAAAAAAAAAAAA414850.21427455396054113No Hit
ACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAAAA402590.2079421301168477No Hit
TCACGTTCTAGCATTCAAGGTCTAAAAAAAAAAAAAAAAAAA395000.2040218122560293No Hit
ATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAA392200.20257558168813847No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAT387790.20029776854371037No Hit
AACTATGCCGACTAGGGATCGGGTGGTCAAAAAAAAAAAAAA382080.19734849120704728No Hit
AATAGGGACGGTCGGGGGCATCAAAAAAAAAAAAAAAAAAAA359590.1857321606813812No Hit
TCACGTTCTAGCATTCAAGGCCAAAAAAAAAAAAAAAAAAAA344380.17787602963223131No Hit
TAATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAA343260.17729753740507498No Hit
CACGTTCTAGCATTCAAGGTCAAAAAAAAAAAAAAAAAAAAA342300.17680168692465525No Hit
ATGCCGACTAGGGATCGGGTGGCGAAAAAAAAAAAAAAAAAA340910.1760837367498809No Hit
AACTATGCCGACTAGGGATCGGGTGGCAAAAAAAAAAAAAAA339630.17542260277598792No Hit
ACGTTCTAGCATTCAAGGTCCCTAAAAAAAAAAAAAAAAAAA336700.17390922578887358No Hit
CACGTTCTAGCATTCAAGGTCCGAAAAAAAAAAAAAAAAAAA334910.17298467124725767No Hit
TCACGTTCTAGCATTCAAGGTCAAAAAAAAAAAAAAAAAAAT331050.1709909391072367No Hit
ATGCCGACTAGGGATCGGGTGGTAAAAAAAAAAAAAAAAAAA327470.16914183002400485No Hit
CACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAT325050.16789187360461347No Hit
AAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAA316820.1636409887568486No Hit
ACGTTCTAGCATTCAAGGTCCCGAAAAAAAAAAAAAAAAAAA315250.16283006661699553No Hit
TTCGGTGCCCGAGTTGTAATTTGAAAAAAAAAAAAAAAAAAA300480.15520120037137136No Hit
CCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAA299690.15479315674685928No Hit
ATGATTAATAGGGACGGTCGGGGGCATCAAAAAAAAAAAAAA291620.15062491364583105No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAA284410.14690086993351212No Hit
ACGTTCTAGCATTCAAGGTCACAAAAAAAAAAAAAAAAAAAA284380.14688537460599901No Hit
CACGTTCTAGCATTCAAGGTCAAAAAAAAAAAAAAAAAAATC276400.14276361748751012No Hit
ATGCCGACTAGGGATCGGGTGGTCAAAAAAAAAAAAAAAAAA272680.14084219687588373No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAA272650.1408267015483706No Hit
AAATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAA271010.13997962364432026No Hit
AATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAAAAATCG269790.13934948032545352No Hit
AGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAA255510.13197370442921025No Hit
CCACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAA252610.130475822769609No Hit
ACTATGCCGACTAGGGATCGGGTGGTCAAAAAAAAAAAAAAA251360.12983018412322916No Hit
TCACGTTCTAGCATTCAAGGACAAAAAAAAAAAAAAAAAAAA250810.12954610311882206No Hit
AATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAA249290.12876100652482414No Hit
TAATGATTAATAGGGACGGTCGGGGGCATCAAAAAAAAAAAA243720.1258840407165556No Hit
CACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAT242310.12515576032343914No Hit
CACGTTCTAGCATTCAAGGTCTAAAAAAAAAAAAAAAAAAAA235430.12160216521376449No Hit
ACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAAT234370.12105466364163439No Hit
ACACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAA233530.12062079447126715No Hit
TTTCGGTGCCCGAGTTGTAATTTGAAAAAAAAAAAAAAAAAA231770.11971173525716433No Hit
ATGATTAATAGGGACGGTCGGGGGCATAAAAAAAAAAAAAAA230490.11905060128327137No Hit
ATGCCGACTAGGGATCGGGTGGCAAAAAAAAAAAAAAAAAAA226250.11686059499475096No Hit
TCACGTTCTAGCATTCAAGGTCGCAAAAAAAAAAAAAAAAAA225050.1162407818942263No Hit
TTCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAA224740.11608066350992412No Hit
GCCAAGGACGTTTTCATTAATCAAGAACAAAAAAAAAAAAAA221250.11427804040923159No Hit
CACGTTCTAGCATTCAAGGCCAAAAAAAAAAAAAAAAAAAAT220030.11364789709036487No Hit
AACTATGCCGACTAGGGATCGGGTGGGGAAAAAAAAAAAAAA217430.1123049687058948No Hit
ACTATGCCGACTAGGGATCGGGTGGCAAAAAAAAAAAAAAAA215240.11117380979743734No Hit
AAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAA209890.10841047639093163No Hit
ATGATTAATAGGGACGGTCGGGGCCAAAAAAAAAAAAAAAAA209100.10800243276641956No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAA206880.10685577853044896No Hit
TCACGTTCTAGCATTCAAGGGCAAAAAAAAAAAAAAAAAAAA205520.1061533236831877No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAAAAAAAAAAAAA203370.10504282521141438No Hit
TCGGTGCCCGAGTTGTAATTTGAAAAAAAAAAAAAAAAAAAA199920.103260862547406No Hit
AGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAA198630.10259456346434202No Hit
TCACGTTCTAGCATTCAAGGTCGAAAAAAAAAAAAAAAAAAA197610.10206772232889606No Hit
ATGATTAATAGGGACGGTCGGGGGAAAAAAAAAAAAAAAAAA194020.10021344813649316No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)