FastQCFastQC Report
Wed 28 Jun 2023
SRR1223698.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1223698.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25664498
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA4150191.6170937767806717No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA4114791.6033004035379925No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAG2032370.7918993778876954No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAGA1741380.6785170705462463No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA1635690.6373356689072975No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTAAAAAAAAAAAAAAAAAAAA1529200.5958425526187966No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAAAAAAAAAAAAAAAAAAA1276690.4974537199208026No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAGAT1261230.4914298343182088No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAA1118900.43597190172977474No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAA1068690.41640791103726243No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAAA1030160.4013949542282105No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAGAT1013310.3948294644220199No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAG986740.3844766416237715No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAA844990.3292447021562627No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAG835120.32539892266741394No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAA796140.31021062636798896No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCAAAAAAAAAAAAAAAAAAAAA748930.291815565611297No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAA724970.28247971185721227No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA722530.2815289821760784No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAGA705060.27472191351648495No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAAAAAAAAAAAAAAA668620.26052331122938777No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAA560500.21839507634242444No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAT558540.21763137545102187No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA540560.2106255887023389No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAG539370.2101619131611302No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAGATC533500.20787470692004184No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAT507810.19786477023630075No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCAAAAAAAAAAAAAAAAAAAA497420.19381637622524314No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAA486100.18940561393408123No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTAAAAAAAAAAAAAAAAAAAAGATC483670.1884587806860668No Hit
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAA443540.1728223945779107No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA437460.1704533632413149No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAA434380.1692532618405394No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAGATC432420.16848956094913684No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCAAAAAAAAAAAAAAAAAA431880.16827915356068918No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAGA419670.16352160872190058No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAAAA405900.15815622031648544No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAA401320.1563716539477998No Hit
AACTTTCAACAACGGATCTCTTGGTTCTCGCAAAAAAAAAAAAAAAAAAA399040.15548326719657637No Hit
TGACCTCAAATCAGGTAGGAGTACCCGCTGAAAAAAAAAAAAAAAAAAAA390280.15206999178398112No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTAAAAAAAAAAAAAAAAAAAAGATG383790.1495412066894899No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAGAT381930.14881647012928131No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAA381160.14851644477908743No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCAAAAAAAAAAAAAAAAAAAA366780.14291337395338882No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTAAAAAAAAAAAAAAAAAAAA358400.13964816299933083No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAGA353500.13773891077082434No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGGAAAAAAAAAAAAAAAAAAAA353420.13770773930586916No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTAAAAAAAAAAAAAAAAAAAG347740.1354945652940494No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA334190.13021489841726108No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAAAAAAAAAAAAAAAAAAA328930.12816537459645616No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAG326810.12733933077514317No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAGAT324940.12661069778181516No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA324590.12647432262263614No Hit
TTGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAA323550.12606909357821844No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGAAAAAAAAAAAAAAAAAAAAA323130.1259054433872036No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTAAAAAAAAAAAAAAAAAAAGATCG314610.12258568236947398No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTAAAAAAAAAAAAAAAAAAAAGA302050.11769176237150636No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAT297610.11596174606649233No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAGA297070.11575133867804466No Hit
AACAACGGATCTCTTGGTTCTCGCATCGAAAAAAAAAAAAAAAAAAAAAA296210.11541624542977617No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAAGA295060.11496815562104508No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAG294280.11466423383773179No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA292470.11395897944312021No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTAAAAAAAAAAAAAAAAAA285550.11126264772449476No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTAAAAAAAAAAAAAAAAAAAAAA283130.11031971090959972No Hit
ACTTTCAACAACGGATCTCTTGGTTCTCGCAAAAAAAAAAAAAAAAAAAA277000.10793119740740692No Hit
ACGATCTGCTGAGATTAAGCCTTTGTTGTCTGAAAAAAAAAAAAAAAAAA276640.10779092581510849No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAG276090.10757662199354143No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAGATG266470.1038282533326777No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGTAAAAAAAAAAAAAAAAAAA256860.10008378110493336No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)