FastQCFastQC Report
Wed 28 Jun 2023
SRR1223699.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1223699.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46043180
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAA10727862.3299563583575242No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAA9024751.9600622719803455No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAA4346830.9440768426507465No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAA3380780.7342629244982645No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTAAAAAAAAAAAAAAAAAAAA2962960.6435176718897347No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAA2541700.5520252945170164No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAAAAAAAA2502870.5435919065538044No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAT2454640.5331169567349605No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAAAAAAAAAAAAAAAAAAA2435200.5288948330675682No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAAA2230290.4843909564891043No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAA1852090.40225066991463226No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAAA1848600.401492685778871No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAAT1813070.3937760163394448No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAA1659370.3603943081255465No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAAA1607820.3491982960342878No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCAAAAAAAAAAAAAAAAAAAAA1510850.3281376308065603No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTAAAAAAAAAAAAAAAAAAAAAATC1497630.3252664129627884No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAA1458410.3167483219013109No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAA1377500.2991756868226738No Hit
TCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAAAAAAAAAAAAAAAA1376980.2990627493583197No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAATC1300880.2825347858249582No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAA1292030.2806126770566238No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGTAAAAAAAAAAAAAAAAAAAAA1216360.2641781041187859No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAA1192870.2590763713540203No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACAAAAAAAAAAAAAAAAAAAAAA1186060.2575973249458443No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTAAAAAAAAAAAAAAAAAAAAAA1176970.25562309119396187No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCAAAAAAAAAAAAAAAAAA1096200.2380808623557278No Hit
TGATGTTGACGCAATGTGATTTCTGCCCAAAAAAAAAAAAAAAAAAAAAT1081970.23499028520619125No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACAAAAAAAAAAAAAAAA1062380.2307355834240815No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAA1019280.22137480512857713No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAAAAAAAAAAAAAAA1018240.22114893019986892No Hit
ACGATCTGCTGAGATTAAGCCTTTGTTGTAAAAAAAAAAAAAAAAAAAAA1011970.21978716500467604No Hit
AACTTTCAACAACGGATCTCTTGGTTCTCGCAAAAAAAAAAAAAAAAAAA1008020.2189292746504477No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAAAAAAAAAAAAAAAAA969160.21048937106429225No Hit
AACAACGGATCTCTTGGTTCTCGCATCGAAAAAAAAAAAAAAAAAAAAAA914080.19852668734001433No Hit
ATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAAAAAAAAAAAAAAA914060.19852234359138532No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGAAAAAAAAAAAAAAAAAAAAA903550.19623970368684351No Hit
TTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAAAAAAA891310.19358132952589285No Hit
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAAAA867600.18843181552620825No Hit
TTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAAAATCG863490.18753917518294783No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTAAAAAAAAAAAAAAAAAAAA859570.186687800451663No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTAAAAAAAAAAAAAAA840850.18262205173491491No Hit
ATGAAGAGCTTTTAGTGGGCCATTTTTGGAAAAAAAAAAAAAAAAAAAAA836670.18171420827145301No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTAAAAAAAAAAAAAAAAAAAAATCG798200.17335900778356317No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCAAAAAAAAAAAAAAAAAAAA796180.17292028917203373No Hit
TGACCTCAAATCAGGTAGGAGTACCCGCTGAAAAAAAAAAAAAAAAAAAA795220.17271178923784153No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAA766060.1663786037367532No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAAAAAAT755970.16418718255342052No Hit
ACTTTCAACAACGGATCTCTTGGTTCTCGCAAAAAAAAAAAAAAAAAAAA753670.163687651461085No Hit
TTGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAA727190.15793652827628327No Hit
TTACGATCTGCTGAGATTAAGCCTTTGTTGTAAAAAAAAAAAAAAAAAAA715980.15550185716972634No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAAAAAAAAAAAAAAAA692410.15038274941044472No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAA688410.14951399968464385No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAAA683710.14849321875682783No Hit
ACGTGGGTTAGTCGATCCTAAGAGATGGGGAAAAAAAAAAAAAAAAAAAA680400.1477743283587276No Hit
ACGATCTGCTGAGATTAAGCCTTTGTTGTCTGAAAAAAAAAAAAAAAAAA669070.14531359476039665No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAAAAAAAAAAAAAAAAAAA668450.14517893855289754No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAAAAAAAAAAAAAATC654980.14225342385126308No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTAAAAAAAAAAAAAAAAAAAAAAAA650760.1413368928905432No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCAAAAAAAAAAAAAAAAAAAA644420.1399599245751488No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAAA638920.1387653937021726No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAAAAAAA626120.13598539457960984No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCAAAAAAAAAAAAAAA604980.13139405227875225No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAAAAAAAAAAAAAAAAAAA591900.12855324067538343No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAAAAAAAAAAAAAAAAAAA572830.12441147635762777No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAAAAAA525870.11421235457672559No Hit
ATGAAGAGCTTTTAGTGGGCCATTTTTGGTAAAAAAAAAAAAAAAAAAAA522710.11352604229334291No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTAAAAAAAAAAAAAAAAAAAAAAAT511570.11110657430698748No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAAAAAAA504720.1096188404015535No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAAAAAAAAAAAAAA503470.10934735611224072No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAAAAAAAAAAAAAAAAAAAT480730.1044085139210628No Hit
ACGATCTGCTGAGATTAAGCCTTTGTTGTCTAAAAAAAAAAAAAAAAAAA475530.10327913927752165No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTAAAAAAAAAAAAAAAAAA468070.10165892103890305No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTAAAAAAAAAAAAAAAAAAAAAT466690.10135920238350175No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGTAAAAAAAAAAAAAAAAAAA460530.1000213278057684No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)