FastQCFastQC Report
Sat 2 Sept 2023
SRR12305269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12305269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77368145
Total Bases11.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC11718921.5146957446116873Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCC4160200.5377148437512622Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC3466490.44805132655048147Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTC3380590.4369485658470938Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG3161000.40856608362524394Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTCT1907090.24649550535301062Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA1659260.21446294208036137Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTCACTCTTCGGCCCCTGGGAAGAGATCGGAAGAGCACACGTCTGAACT1513090.19557015358194255Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATACAATCTCGTA1475950.1907697282906292TruSeq Adapter, Index 22 (97% over 37bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT1412320.18254541323176354Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1054630.13631320745766878Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC981930.12691657529077374No Hit
CGACTGCATAATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC949090.12267193429543904Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACT902640.11666817137725094Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTC834690.10788548697916953Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTACGGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCA828200.1070466404487273TruSeq Adapter, Index 22 (97% over 37bp)
CTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAAC817870.10571146561675997Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC805900.1041643172393496Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)