FastQCFastQC Report
Sat 2 Sept 2023
SRR12305270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12305270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66790766
Total Bases10 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC11517021.7243431524651176Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC10764301.6116449390623848No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC7294521.0921449830355292No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCAGATCGGAAGAGCACACGTCTGA5575090.8347096962475322Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTC4980880.7457438053637534Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGCTTCTGTCGTGAGTGGCACACGCAAGATCGGAAGAGCACACGTCTG4681400.7009052718455122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC4171930.624626763525964Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCC3584720.5367089217093274Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT3152340.4719724280449186No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2599100.38914061862982674No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT2556140.38270859178348093No Hit
GCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAA2323420.34786545193986845Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTCT2067780.3095907000078424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG1920460.28753375878336235Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1874640.2806735290324414No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1568750.23487528201128882No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATCTCGTA1449500.21702101754604822TruSeq Adapter, Index 1 (97% over 37bp)
AAATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACT1421770.2128692460272128Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGT1414540.2117867610621504No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTAGATCGGAAGAGCACACGTC1400660.20970862948330316No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC1371760.2053816840489597No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT1357240.20320773084111657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC1332300.19947368173618493Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTTGGTTATCTAGCTGTATGAAGATCGGAAGAGCACACGTCTGAACTC1181990.17696907383873992Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA1145790.17154916294866268Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1131060.16934376826880532Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1122140.16800825431467575No Hit
ACGGTCTGTATTCGTACTGAAAATCAGATCGGAAGAGCACACGTCTGAAC1076430.16116449390623847Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1058490.15847849386844884No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1017470.15233692633499668No Hit
TGTCACTCTTCGGCCCCTGGGAAGAGATCGGAAGAGCACACGTCTGAACT1003910.1503067055706473Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA986230.1476596330696372Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCA978880.1465591815491381Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCGCCGAATCCCGGGGCCGAGGAAAGATCGGAAGAGCACACGTCTGAA973600.14576865310992243Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGGCTTCTGTCGTGAGTGGCACACGCAGATCGGAAGAGCACACGTCTG960780.14384922610409948Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGACTCTAGATAACCTCGGGAGATCGGAAGAGCACACGTCTGAACT956760.14324734649696935Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA927560.13887548467403413Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTGACTCTAGATAACCTCGGAGATCGGAAGAGCACACGTCTGAACTC918110.1374606184333924Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT910930.13638561953309533Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC856090.12817490369851425No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG847980.12696066399358258No Hit
AATGGATTTTTGGAAGTAGGAGATCGGAAGAGCACACGTCTGAACTCCAG847270.12685436187391533Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTC845540.12659534403303593No Hit
AAGGGCTTCTGTCGTGAGTGGCACACGCAAGATCGGAAGAGCACACGTCT827530.12389886350457488Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTTTCTCTTCAGATCGTATAAATCTTTCGCAGATCGGAAGAGCACACGT817250.12235972858882918No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT811730.12153326703874005No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC810000.12127424919786067No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTGAAC791700.11853434949376086Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAATACAAGCTTGGGCTGCAGGTCAGATCGGAAGAGCACACGTCTGAACT786060.11768992138823502Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCA764480.11445893583553152No Hit
GGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCTGAA763800.11435712535472344Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTCACTCTTCGGCCCCTGGGAAGGTCTGAAGATCGGAAGAGCACACGTC762970.11423285667961945No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC750260.11232989901628018Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGATCGGAAGAGCACACGTCTGA742940.11123393913464025Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG728090.10901057789934614No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC725790.10866621892014233No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT712180.10662851209102767Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA678480.10158290443921544No Hit
CGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACTC674400.10097204155436695Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)