FastQCFastQC Report
Sat 2 Sept 2023
SRR12305271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12305271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74463814
Total Bases11.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC12416141.6674058623964656Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA12323901.655018637643245No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC10604031.4240514191228508No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTA6944680.9326248048481642TruSeq Adapter, Index 7 (97% over 37bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG5468240.7343486327466385Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTCT5018800.6739917995605221Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC4758870.6390849117666737No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4335390.5822143356771922No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC3436870.4615490149349589No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2712380.3642547774950125No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT2120890.28482156447157003No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT1860860.24990124733605507Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1726820.23190055776621918No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1711590.22985526903040449No Hit
AAATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACT1676860.22519125866961365Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAATACAAGCTTGGGCTGCAGGTCGACCAGATCGGAAGAGCACACGTCTG1665290.2236374838387945Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCAGATCGGAAGAGCACACGTCT1635210.219597937865498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTC1567490.21050358768891422Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1526610.20501367281563096No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG1519340.2040373596764732No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGAGATCGGAAGAGCACACG1508840.20262727880148604No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1385920.18611993202496988No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1292190.17353261008091794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG1245960.16732422542847455Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTCTG1221800.16407969648183746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1135470.15248614582110984Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTCTTAGCGGTGGATCACTCGGCAGATCGGAAGAGCACACGTCTGAA1129950.1517448461611166Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1115760.14983922257863397Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTGA1078640.14485425095201274Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1033500.13879224612373467Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGA989960.13294511076212132Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCC948550.12738402037800534Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT925750.12432213047803325No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAGATCGGAAGAGCACACGTCTG912590.12255482911471603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTCTTAGCGGTGGATCACTCGGCTAGATCGGAAGAGCACACGTCTGA893980.12005562863057215Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC848650.11396810805312765Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT844150.11336378767813317No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT824790.11076386713149021No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTC819010.10998765118316395No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG790090.10610388557319936Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAGATCGGAAGAGCACACGTCT757630.1017447212682391Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGAAGATCGGAAGAGCACACGTCTGAA750990.10085301298158056Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)