FastQCFastQC Report
Sat 2 Sept 2023
SRR12305272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12305272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59502885
Total Bases8.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC10639961.7881418690875241Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC9159611.5393556127572638Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCC8248721.386272279066805Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCA6290721.057212604061131Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTCT4276170.7186491881864215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC3346030.5623307172416934No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC2781930.4675285912607431No Hit
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA2742600.4609188277173451Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG2200300.3697803896399309Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTC1998630.33588791535065904Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC1980730.332879657851884No Hit
GAATACAAGCTTGGGCTGCAGGTCAGATCGGAAGAGCACACGTCTGAACT1952530.3281403918482272Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTCAATCTCGTA1825400.3067750412437985TruSeq Adapter, Index 5 (97% over 38bp)
CTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTC1375010.23108291303858627Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAATACAAGCTTGGGCTGCAGGTAGATCGGAAGAGCACACGTCTGAACTC1335980.22452356721863148Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTCC1312100.22051031643255617Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTCTG1296170.21783313531772452Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT1292180.21716257959593055No Hit
ATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAACT1288040.21646681501241494Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1283800.21575424452108496No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1268060.21310899463110067Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCATAATTTGTGGTAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG1232670.20716138385558952Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGGGCTTCTGTCGTGAGTGGCACACGCAAGATCGGAAGAGCACACGTCT1213520.20394305250913464Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC1207890.20299687989918472Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGGATTTTTGGAAGTAGGAGATCGGAAGAGCACACGTCTGAACTCCAG1182250.1986878451355762Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGGGCTTCTGTCGTGAGTGGCACACGCAGATCGGAAGAGCACACGTCTG1138680.1913655111008483Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCA1074040.1805021722896293Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTGATCATCGACACTTCGAACAAGATCGGAAGAGCACACGTCTGAACTC1058590.17790565953230672Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGA1006840.16920860223836207Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTCACTCTTCGGCCCCTGGGAAGGTCTGAGATCGGAAGAGCACACGTCT999670.1680036186480706Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGGATCACTCGGCTCGTGCGTCGATGAGATCGGAAGAGCACACGTCT998260.16776665534788776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACTC953400.1602275251023543Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA918610.15438074977372945Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAG915830.15391354553648953Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTCACTCTTCGGCCCCTGGGAAGAGATCGGAAGAGCACACGTCTGAACT871240.1464197912420549Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGAAAGGATGAACGAACTTGGCCTGACTAGATCGGAAGAGCACACGTCT853270.14339976960780978Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC849000.1426821573441355Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC832030.13983019478803424Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA817520.13739165756416685Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA814710.1369194115545826Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAGATCGGAAGAGCACACGTCTG803440.13502538574390133Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGGATCACTCGGCTCGTGCGTCGAGATCGGAAGAGCACACGTCTGAA802450.1348590072565389Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGACTGGTGTGCGGACACGTAGATCGGAAGAGCACACGTCTGAACTCC780420.1311566657650297Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT761590.12799211332358088Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG737420.12393012540484383No Hit
TGCATAATTTGTGGTAGTGGGAAGATCGGAAGAGCACACGTCTGAACTCC699280.11752035216443706Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAGAATTTTTGAGCGGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG605020.10167910345859701Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)