FastQCFastQC Report
Sat 2 Sept 2023
SRR12305273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12305273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73960058
Total Bases11 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTA7222580.9765514245540478TruSeq Adapter, Index 7 (97% over 37bp)
AGGGCTTCTGTCGTGAGTGGCACACGCAAGATCGGAAGAGCACACGTCTG6757540.9136742429271757Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCTTCTGTCGTGAGTGGCACACGCAGATCGGAAGAGCACACGTCTGA5168950.6988839841093689Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC4738490.6406822990863528No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3915380.529391147854427No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG3850300.5205918037543994No Hit
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC3628100.4905485606839302Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGATTTTTGGAAGTAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT3319600.4488368573210151Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCC2883090.3898171632044961Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCA2852780.38571900525010405Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATGGATTTTTGGAAGTAGGAGATCGGAAGAGCACACGTCTGAACTCCAG2829310.3825456707997714Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA2255920.3050186899528932Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC2076320.2807353125656013Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTCT1737100.2348700159212963Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG1726170.233392191228406No Hit
AATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTC1582700.21399388302264447Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1525590.20627214759620657No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACG1459610.19735111619301327No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGATCGGAAGAGCACACGTCTGA1364840.18453744316966328Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT1274130.1722727150917053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1093130.14780004634393337No Hit
GCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1081060.1461680844003665Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAAGAATTTTTGAGCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1073780.14518376932587046Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1056370.142829796050187Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1022120.1381989181241583No Hit
CATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT1013590.13704559290637658Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1010960.13668999556490344Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG857380.115924733320247No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG843020.11398314479418067Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA784470.10606670968267763No Hit
CTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTC768660.10392906939039989Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACTC757150.10237282399102499Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAGAATTTTTGAGCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGT739820.10002966736451181Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)