FastQCFastQC Report
Sat 2 Sept 2023
SRR12305274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12305274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52698829
Total Bases7.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGCTTCTGTCGTGAGTGGCACACGCAAGATCGGAAGAGCACACGTCTG6752501.2813377693838321Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCC6525801.2383197357193647Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCA5540411.051334556219456Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC4881740.9263469592464759Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCTTCTGTCGTGAGTGGCACACGCAGATCGGAAGAGCACACGTCTGA4793360.9095761881160584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTC3427930.6504755542101325Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC3325650.6310671533137862Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2458080.4664392068370248No Hit
CTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTC1909270.3622983728917392Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG1845150.35013111961178495Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCA1427540.2708864745362748Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1422180.26986937413732665No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGATCGGAAGAGCACACGTCTGA1396640.2650229666393536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAGTAGGAGATCGGAAGAGCACACGTCTGAACTCCAG1249170.23703942264068142Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1151350.21847734035987781Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1081200.20516584913110686No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1023370.19419217075961973Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCATAATTTGTGGTAGTGGAGATCGGAAGAGCACACGTCTGAACTCCAG1015410.19268170076416688Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA981470.18624132995440942Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCATAATTTGTGGTAGTGGGAAGATCGGAAGAGCACACGTCTGAACTCC979140.18579919489292637Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCAG965050.18312551119494513Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACT948390.17996415062657276Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGGCTTCTGTCGTGAGTGGCACACGCAAGATCGGAAGAGCACACGTCT930080.17648969012195698Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT908650.17242318610153556Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT818020.15522546051260455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA779340.1478856389769116Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG768930.14591026301552165No Hit
AAGGGCTTCTGTCGTGAGTGGCACACGCAGATCGGAAGAGCACACGTCTG749600.14224224982304637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTA738800.14019286842218068TruSeq Adapter, Index 16 (97% over 37bp)
AATGGATTTTTGGAAGTAGGAGAGATCGGAAGAGCACACGTCTGAACTCC735040.13947938008262006Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC717560.13616241833381157Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAACT703710.13353427644473845Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC687310.13042225283601655Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTCT663850.1259705410152472Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACAAGATCGGAAGAGCACACGTCTGAACTC648420.12304258221752896Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCATAATTTGTGGTAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCA647330.12283574650207123Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGAACTC641890.12180346550015372Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAATACAAGCTTGGGCTGCAGGTCAGATCGGAAGAGCACACGTCTGAACT618670.11739729548829254Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGGTGTGCGGACACGTAGATCGGAAGAGCACACGTCTGAACTCC600800.11400632830000834Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA595680.11303476970996831Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC594590.11282793399451058No Hit
GAAGAATTTTTGAGCGGGTAAGATCGGAAGAGCACACGTCTGAACTCCAG542630.10296813236590134Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)