FastQCFastQC Report
Sat 2 Sept 2023
SRR12305275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12305275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49984634
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC8638581.7282471249064262Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCTTCTGTCGTGAGTGGCACACGCAAGATCGGAAGAGCACACGTCTG6025331.2054364547312681Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC4729440.9461787796625659No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCAGATCGGAAGAGCACACGTCTGA4506990.9016751027925903Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTC3413650.6829398810842549Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG3015650.6033154108920753Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA2884000.5769773166689587No Hit
TGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCC2385170.4771806471564841Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTCT2351600.4704645831757015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTC1643090.3287190219298195Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACT1628270.3257541107533167Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1351540.2703910965918046Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1278410.2557606003476989Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCTTCTGTCGTGAGTGGCACACGTAGATCGGAAGAGCACACGTCTGA1253150.2507070472897731Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT1182790.2366307213532863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1160880.23224737426305853No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC1106720.22141204434946948No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA1105940.22125599639281143Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1005220.20110580383563476No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC968510.19376154679856214Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTCTG936600.18737758487938513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGCTTCTGTCGTGAGTGGCACACGCAAGATCGGAAGAGCACACGTCT935990.18725554737481923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA901230.1803014102293917Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA811430.1623358890654276Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAACT787760.15760043376530475Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGATCGTATAGTGGTTAGTACTCTGTAGATCGGAAGAGCACACGTCTG755740.1511944650830093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGCTTCTGTCGTGAGTGGCACACGCAGATCGGAAGAGCACACGTCTG752320.15051025481150868Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGAAAGGATGAACGAACTTGGCCTGACTAGATCGGAAGAGCACACGTCT730650.14617492247717567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT707330.14150948869606608Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG698550.13975294887624864No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGTA688140.1376703088393125TruSeq Adapter, Index 23 (97% over 37bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC686010.1372441778807463No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA661430.13232666663118908No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC657820.13160444467793841No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGT650200.1300799761782791No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC637340.1275071855082504No Hit
TGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCA611950.12242762445754829Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAA609530.12194347566894259Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAATGATGAACAATACTGCTGGAGCAGATCGGAAGAGCACACGTCTGAA598660.11976880734987476Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAAGTAGGAGATCGGAAGAGCACACGTCTGAACTCCAG559940.11202242673218332Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT531620.1063566855365991No Hit
AAGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTC527500.10553243222707201No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)