FastQCFastQC Report
Sat 2 Sept 2023
SRR12305276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12305276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61068900
Total Bases9.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC18519333.0325304696826048No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA17852882.92339963549368No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC17116542.8028243508561643No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA16395162.68469875828777No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC15764772.5814727299820364No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACGT12140891.988064301141825No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA11711671.9177797536880474No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA11354201.8592442306968031No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC10700991.7522814394888397No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT8759341.4343372813330517No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC8661501.4183160332018425No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA7959531.3033688178434522No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG7568411.2393231251913823No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCAAGATCGGAAGAGCACACGTCTG4725990.773878357068819Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT4037450.6611302970906632No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC4016790.657747233043333No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC3854930.6312427438516167No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCAGATCGGAAGAGCACACGTCTGA2985570.48888550473317843Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC2922590.47857256312132695No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC2879560.4715264234332041No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCAGATCGGAAGAGCACACGTCTG2824870.462570964926501Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG2806010.4594826499249209No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC2597860.4253981977733347No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGAGATCGGAAGAGCACA2565130.4200386776247812No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGAGATCGGAAGAGCACAC2347410.38438714304662436No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA2304590.37737539074717247No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG2285540.37425596334631867No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC2276920.37284444291611607No Hit
CTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAGATCGGAAGAGCACACG2213580.36247255149511454No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2125600.3480658731367357No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2071650.33923158923772984No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGAGATCGGAAGAGCAC1852180.3032934930873161No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA1788970.2929428890973966No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1718200.28135433911532715No Hit
TAATCCACCTATAACTTCTCTGTTAACCCAACAGATCGGAAGAGCACACG1672230.27382677598581273No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT1655930.27115765962707694No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGAGATCGGAAGAGCACACGTCTGA1594820.26115092952386565Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAG1546040.2531632303840416No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG1468240.24042352162884872No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1456080.23843232807533787No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1290160.2112630160359856No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGCCAGAAGATCGGAAGAGCACACG1262830.20678774302468195No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT1250290.2047343246726239No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAGATCGGAAGAGCACAC1249600.2046213375384197No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT1182250.19359281074327522Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGGAGATCGGAAGAGCACACG1110470.1818388737966461No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAACAGATCGGAAGAGCACAC1103350.1806729775712351No Hit
ACATATACTAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGT1102900.18059929030979763No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC1095480.17938426924342832No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCGAGATCGGAAGAGCACACGTCTG1041470.17054016037623076Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1034700.16943157646527118No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1006080.16474506663784674No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG988670.16189418836756517No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC957400.15677374244500883No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC956110.15656250562888802No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA935640.15321055398083147No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGAGATCGGAAGAG931940.15260468094234544No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAGATCGGAAGAG905370.14825385752813625No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG882020.14443030740687976Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGAGATCGGAAGAGC858050.14050523261430942No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGAGATCGGAAGAGCACA840200.13758230457728893No Hit
AGGGCTTCTGTCGTGAGTGGCACACGCAGAGATCGGAAGAGCACACGTCT814260.13333464332909223Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATGAGATCGGAAGAGCACAC796400.13041007779737313No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC783780.12834355948772616No Hit
TTTCGCCGAATCCCGGGGCCGAGGAAGATCGGAAGAGCACACGTCTGAAC780940.12787851099332065Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGAGATCGGAAGAGCACAC776730.12718912572520547No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA761760.12473779616138493No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG754100.12348347522224898No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGAGATCGGAAGAGCA744920.12198025508892414No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCACA743050.12167404358028391No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT714520.11700227120514699Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG710970.1164209605871401Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACACGT702820.1150864024077722No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACACG650470.10651411766054407No Hit
GAAGAATTTTTGAGCGGGTATAGGTTGCAGATCGGAAGAGCACACGTCTG631960.10348311497341528Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCA627300.1027200424438626No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGAGATCGGAAGAGCACAC613100.10039479997183509No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)