FastQCFastQC Report
Sat 2 Sept 2023
SRR12305277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12305277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68217629
Total Bases10.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC17110262.508187436417645Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCC5773540.8463413467507057Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACTC5733710.8405026800330454Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAACTC2467190.36166457793483264Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTCT2303590.3376825072592306Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG1839600.2696663643938725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACTGGAATCTCGTA1799850.2638394248501366TruSeq Adapter, Index 23 (97% over 38bp)
CTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTC1714250.25129134875092185Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATTTTTGGAAGTAGGAGTAGATCGGAAGAGCACACGTCTGAACT1580370.23166592318827148Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA1403640.2057591300923109Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1377570.20193753728966454Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGTGACTCTAGATAACCTCGGGAGATCGGAAGAGCACACGTCTGAACT1265710.18554001634973272Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1157200.16963357081788932Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC1105350.1620328962180729No Hit
GGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTCTG1041470.15266874783935983Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1040600.1525412148229309Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACTCCA972970.1426273551665069Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAACT759280.11130260771742742Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT750330.109990630134624Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC708170.10381040947641262Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)